LOCUS       BBa_J11011               954 bp    DNA     linear       10-May-2024
DEFINITION  variant of J11005 uncoupled from CI(ts) production
ACCESSION   BBa_J11011
VERSION     BBa_J11011.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     RBS             91..105
                     /label=RBS-1\Strong
     RBS             98..102
                     /label=RBS
     misc_feature    91..105
                     /label=BBa_B0030
     RBS             91..105
                     /label=BBa_B0030
     CDS             112..786
                     /label=mrfp1
     misc_feature    793..817
                     /label=Help:Barcodes
     CDS             112..792
                     /label=BBa_E1010
     stem_loop       837..880
                     /label=stem_loop
     misc_feature    826..905
                     /label=BBa_B0010
     terminator      826..905
                     /label=BBa_B0010
     stem_loop       921..940
                     /label=T7 TE
     misc_feature    947..947
                     /label=stop
     polyA_site      941..954
                     /label=polya
     misc_feature    914..954
                     /label=BBa_B0012
     terminator      914..954
                     /label=BBa_B0012
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag attaaagagg agaaatacta gatggcttcc
      121 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      181 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      241 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      301 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      361 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      421 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      481 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      541 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      601 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
      661 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
      721 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
      781 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
      841 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      901 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//