LOCUS BBa_J11015 2899 bp DNA linear 01-Jun-2024 DEFINITION -- No description -- ACCESSION BBa_J11015 VERSION BBa_J11015.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 64..67 /label=conserved RBS 58..71 /label=BBa_B0031 CDS 78..797 /label=GFP protein CDS 78..797 /label=BBa_E0040 stem_loop 817..860 /label=stem_loop misc_feature 806..885 /label=BBa_B0010 terminator 806..885 /label=BBa_B0010 stem_loop 901..920 /label=T7 TE misc_feature 927..927 /label=stop polyA_site 921..934 /label=polya misc_feature 894..934 /label=BBa_B0012 terminator 894..934 /label=BBa_B0012 misc_feature 1..934 /label=BBa_J11008 misc_feature 951..967 /label=OR1 misc_feature 975..991 /label=OR2 promoter 990..995 /label=-35 misc_feature 1001..1007 /label=mutate to obliterate OR3 promoter 1013..1018 /label=-10 promoter 943..1024 /label=BBa_I12007 RBS 1033..1047 /label=RBS-1\Strong RBS 1040..1044 /label=RBS misc_feature 1033..1047 /label=BBa_B0030 RBS 1033..1047 /label=BBa_B0030 CDS 1054..1728 /label=mrfp1 misc_feature 1735..1759 /label=Help:Barcodes CDS 1054..1734 /label=BBa_E1010 stem_loop 1779..1822 /label=stem_loop misc_feature 1768..1847 /label=BBa_B0010 terminator 1768..1847 /label=BBa_B0010 stem_loop 1863..1882 /label=T7 TE misc_feature 1889..1889 /label=stop polyA_site 1883..1896 /label=polya misc_feature 1856..1896 /label=BBa_B0012 terminator 1856..1896 /label=BBa_B0012 misc_feature 943..1896 /label=BBa_J11009 misc_feature 1..1896 /label=BBa_J11010 misc_feature 1905..1923 /label=TetR 1 misc_feature 1905..1958 /label=BBa_R0040 promoter 1924..1929 /label=-35 misc_feature 1930..1948 /label=TetR 2 promoter 1947..1952 /label=-10 promoter 1905..1958 /label=BBa_R0040 RBS 1967..1981 /label=RBS-1\Strong RBS 1974..1978 /label=RBS misc_feature 1967..1981 /label=BBa_B0030 RBS 1967..1981 /label=BBa_B0030 CDS 1988..2698 /label=cI857 misc_feature 2699..2731 /label=LVA misc_feature 2186..2186 /label=G->A (857) misc_feature 2339..2339 /label=G->A (ind-) misc_feature 2738..2762 /label=barcode from LacI-LVA (BBa_C0012) CDS 1988..2762 /label=BBa_J06503 stem_loop 2782..2825 /label=stem_loop misc_feature 2771..2850 /label=BBa_B0010 terminator 2771..2850 /label=BBa_B0010 stem_loop 2866..2885 /label=T7 TE misc_feature 2892..2892 /label=stop polyA_site 2886..2899 /label=polya misc_feature 2859..2899 /label=BBa_B0012 terminator 2859..2899 /label=BBa_B0012 misc_feature 1905..2899 /label=BBa_J11013 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggaaac ctactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 121 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 181 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 241 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 301 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 361 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 421 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 481 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 541 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 601 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 661 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 721 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 781 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 901 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aggcaaccat tatcaccgcc 961 agaggtaaaa tagtcaacac gcacggtgtt agatatttat aaatagtggt gatagattta 1021 acgttactag agattaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa 1081 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc 1141 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc 1201 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc 1261 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa 1321 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag 1381 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc 1441 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt 1501 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac 1561 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg 1621 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc 1681 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga 1741 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 1801 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1861 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 1921 agattgacat ccctatcagt gatagagata ctgagcacta ctagagatta aagaggagaa 1981 atactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg 2041 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc 2101 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt 2161 aaatgcttat aacgccgcat tgcttacaaa aattctcaaa gttagcgttg aagaatttag 2221 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact 2281 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctaa 2341 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag 2401 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa 2461 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg 2521 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga 2581 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga 2641 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc 2701 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc 2761 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2821 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2881 gggcctttct gcgtttata //