LOCUS       BBa_J11018              1896 bp    DNA     linear       13-Jun-2024
DEFINITION  -- No description --
ACCESSION   BBa_J11018
VERSION     BBa_J11018.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     CDS             79..753
                     /label=mrfp1
     misc_feature    760..784
                     /label=Help:Barcodes
     CDS             79..759
                     /label=BBa_E1010
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     misc_feature    1..921
                     /label=BBa_J11016
     misc_feature    938..954
                     /label=OR1
     misc_feature    962..978
                     /label=OR2
     promoter        977..982
                     /label=-35
     misc_feature    988..994
                     /label=mutate to obliterate OR3
     promoter        1000..1005
                     /label=-10
     promoter        930..1011
                     /label=BBa_I12007
     misc_feature    1026..1029
                     /label=conserved
     RBS             1020..1033
                     /label=BBa_B0031
     CDS             1040..1759
                     /label=GFP protein
     CDS             1040..1759
                     /label=BBa_E0040
     stem_loop       1779..1822
                     /label=stem_loop
     misc_feature    1768..1847
                     /label=BBa_B0010
     terminator      1768..1847
                     /label=BBa_B0010
     stem_loop       1863..1882
                     /label=T7 TE
     misc_feature    1889..1889
                     /label=stop
     polyA_site      1883..1896
                     /label=polya
     misc_feature    1856..1896
                     /label=BBa_B0012
     terminator      1856..1896
                     /label=BBa_B0012
     misc_feature    930..1896
                     /label=BBa_J11017
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
      121 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      181 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      241 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      301 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      361 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      421 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      481 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      541 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      601 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      661 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      721 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      781 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagagg caaccattat caccgccaga ggtaaaatag
      961 tcaacacgca cggtgttaga tatttataaa tagtggtgat agatttaacg ttactagagt
     1021 cacacaggaa acctactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
     1081 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1141 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1201 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1261 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1321 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1381 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1441 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1501 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1561 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1621 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1681 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1741 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1801 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1861 ctggctcacc ttcgggtggg cctttctgcg tttata
//