LOCUS BBa_J11021 5588 bp DNA linear 09-May-2024 DEFINITION Tunable Temperature Sensor v2 (using LacI ts + other parts from Registry 7.05) ACCESSION BBa_J11021 VERSION BBa_J11021.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2364 /label=LacI(ts)-LVA misc_feature 2039..2039 /label=TS mutation (G->A) misc_feature 2326..2364 /label=LVA misc_feature 2365..2389 /label=barcode from LacI (BBa_C0012) CDS 1237..2389 /label=BBa_J06501 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 CDS 2535..2622 /label=end of LacI coding region (inactive) misc_feature 2535..2734 /label=BBa_R0010 misc_feature 2623..2660 /label=CAP binding site promoter 2671..2676 /label=-35 promoter 2695..2700 /label=-10 misc_feature 2700..2734 /label=LacI binding site misc_feature 2707..2707 /label=start promoter 2535..2734 /label=BBa_R0010 misc_feature 2749..2752 /label=conserved RBS 2743..2756 /label=BBa_B0031 misc_feature 3393..3398 /label=2 CDS 2763..3398 /label=cI 434 CDS 2763..3398 /label=BBa_C0056 stem_loop 3418..3461 /label=stem_loop misc_feature 3407..3486 /label=BBa_B0010 terminator 3407..3486 /label=BBa_B0010 stem_loop 3502..3521 /label=T7 TE misc_feature 3528..3528 /label=stop polyA_site 3522..3535 /label=polya misc_feature 3495..3535 /label=BBa_B0012 terminator 3495..3535 /label=BBa_B0012 misc_feature 3599..3612 /label=OR1 434 misc_feature 3552..3568 /label=OR1 lambda misc_feature 3576..3592 /label=OR2 lambda promoter 3614..3619 /label=-10 promoter 3591..3596 /label=-35 promoter 3544..3625 /label=BBa_I12006 RBS 3634..3646 /label=RBS-3\Weak RBS 3640..3643 /label=RBS misc_feature 3634..3646 /label=BBa_B0032 RBS 3634..3646 /label=BBa_B0032 CDS 3653..4372 /label=GFP protein CDS 3653..4372 /label=BBa_E0040 stem_loop 4392..4435 /label=stem_loop misc_feature 4381..4460 /label=BBa_B0010 terminator 4381..4460 /label=BBa_B0010 stem_loop 4476..4495 /label=T7 TE misc_feature 4502..4502 /label=stop polyA_site 4496..4509 /label=polya misc_feature 4469..4509 /label=BBa_B0012 terminator 4469..4509 /label=BBa_B0012 CDS 4518..4605 /label=end of LacI coding region (inactive) misc_feature 4518..4717 /label=BBa_R0010 misc_feature 4606..4643 /label=CAP binding site promoter 4654..4659 /label=-35 promoter 4678..4683 /label=-10 misc_feature 4683..4717 /label=LacI binding site misc_feature 4690..4690 /label=start promoter 4518..4717 /label=BBa_R0010 misc_feature 4732..4735 /label=conserved RBS 4726..4739 /label=BBa_B0031 CDS 4746..5420 /label=mrfp1 misc_feature 5427..5451 /label=Help:Barcodes CDS 4746..5426 /label=BBa_E1010 stem_loop 5471..5514 /label=stem_loop misc_feature 5460..5539 /label=BBa_B0010 terminator 5460..5539 /label=BBa_B0010 stem_loop 5555..5574 /label=T7 TE misc_feature 5581..5581 /label=stop polyA_site 5575..5588 /label=polya misc_feature 5548..5588 /label=BBa_B0012 terminator 5548..5588 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttgat 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 2581 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 2641 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 2701 gtgtggaatt gtgagcggat aacaatttca cacatactag agtcacacag gaaacctact 2761 agatgagtat ttcttccagg gtaaaaagca aaagaatcca gcttggactt aaccaggctg 2821 aacttgctca aaaggtgggg actacccagc agtctataga gcagctcgaa aacggtaaaa 2881 ctaagcgacc acgcttttta ccagaacttg cgtcagctct tggcgtaagt gttgactggc 2941 tgctcaatgg cacctctgat tcgaatgtta gatttgttgg gcacgttgag cccaaaggga 3001 aatatccatt gattagcatg gttagagctg gttcgtggtg tgaagcttgt gaaccctacg 3061 atatcaagga cattgatgaa tggtatgaca gtgacgttaa cttattaggc aatggattct 3121 ggctgaaggt tgaaggtgat tccatgacct cacctgtagg tcaaagcatc cctgaaggtc 3181 atatggtgtt agtagatact ggacgggagc cagtgaatgg aagccttgtt gtagccaaac 3241 tgactgacgc gaacgaagca acattcaaga aactggtcat agatggcggt cagaagtacc 3301 tgaaaggcct gaatccttca tggcctatga ctcctatcaa cggaaactgc aagattatcg 3361 gtgttgtcgt ggaagcgagg gtaaaattcg tataataata ctagagccag gcatcaaata 3421 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3481 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 3541 gaggcaacca ttatcaccgc cagaggtaaa atagtcaaca cgcacggtgt tagatattac 3601 aaactttctt gtatagattt aacgttacta gagtcacaca ggaaagtact agatgcgtaa 3661 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa 3721 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac 3781 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac 3841 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt 3901 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga 3961 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat 4021 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata 4081 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt 4141 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca 4201 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac 4261 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt 4321 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag 4381 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 4441 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 4501 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 4561 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 4621 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 4681 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagtcaca caggaaacct 4741 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 4801 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 4861 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 4921 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 4981 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 5041 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 5101 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 5161 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 5221 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 5281 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 5341 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 5401 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc 5461 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 5521 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 5581 cgtttata //