LOCUS       BBa_J119044              926 bp    DNA     linear       13-Jun-2024
DEFINITION  RFP GGA destination vector BsaI
ACCESSION   BBa_J119044
VERSION     BBa_J119044.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=Bba prefix
     misc_feature    23..26
                     /label=BsaI sticky end left
     misc_feature    28..33
                     /label=BsaI cuts left
     stem_loop       34..128
                     /label=TT B0014
     misc_feature    129..134
                     /label=Bsa cuts right
     misc_feature    136..139
                     /label=BsaI sticky end right
     misc_feature    140..227
                     /label=BD18 bicistron
     RBS             157..165
                     /label=leader RBS
     RBS             210..218
                     /label=RFP RBS
     misc_feature    223..225
                     /label=Stop for BD18
     misc_feature    225..227
                     /label=Start RFP
     CDS             225..905
                     /label=RFP optimized for E. coli 
     misc_feature    900..905
                     /label=Double Stop
     misc_feature    906..926
                     /label=BBa suffix
ORIGIN
        1 gaattcgcgg ccgcttctag agcgactgag acctcacact ggctcacctt cgggtgggcc
       61 tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat ccggcttttt
      121 tattatttgg tctcagcggg ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca
      181 attttcgtac tgaaacatct taatcatgcg acggagcgtt tctaatggca agcagcgaag
      241 atgtgatcaa agaatttatg cgtttcaagg tgcgtatgga aggtagcgtt aatggtcatg
      301 aatttgaaat tgaaggtgaa ggcgaaggtc gtccgtatga aggcacccag accgcaaaac
      361 tgaaagttac caaaggtggt ccgctgccgt ttgcatggga tattctgagt ccgcagttcc
      421 agtatggtag caaagcatat gttaaacatc cggcagatat tccggattac ctgaaactga
      481 gctttccgga aggttttaaa tgggaacgtg tgatgaattt tgaagatggt ggtgttgtta
      541 ccgttaccca ggatagcagc ttacaggatg gtgagtttat ctacaaagtt aaactgcgtg
      601 gcaccaactt tccgagtgat ggtccggtta tgcagaaaaa aaccatgggt tgggaagcaa
      661 gcaccgaacg tatgtatccg gaagatggcg cactgaaagg tgaaatcaaa atgcgtctga
      721 agctgaaaga cggtggtcat tatgatgcag aagttaaaac cacctacatg gccaaaaaac
      781 cggttcagct gcctggtgca tataaaaccg atattaaact ggatatcacc agccacaacg
      841 aggattatac cattgtggaa cagtatgaac gtgcagaagg tcgccatagt accggtgcct
      901 aataatacta gtagcggccg ctgcag
//