LOCUS       BBa_J119092             1534 bp    DNA     linear       14-Jun-2024
DEFINITION  pLacI-HSP (from Shigella sonnei)
ACCESSION   BBa_J119092
VERSION     BBa_J119092.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    1..223
                     /label=BBa_S03511
     misc_feature    230..1534
                     /label=Heat Shock Protein (yegD Chaperone
     misc_feature    230..1534
                     /label=BBa_J119091
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcgtgacgg
      241 taaaccgcaa ttactgaaaa tggaaaacga cagcacgctg ctgccttcaa tgctttgcgc
      301 gccaacgcgt gaatcggtaa gcgaatggct gtaccgccat catgatgttc cggcagacga
      361 cgatgaaacg caggcgctgt tacgtcgggc gattcgttat aaccgcgaag aagatatcga
      421 tgttacggcg aaaagcgtgc agttcggtct ttcctcactg gcacagtaca ttgatgatcc
      481 agaagaagtg tggtttgtga aatcaccaaa atcgttcctc ggtgccagcg gcttaaaacc
      541 gcagcaggta gcgctgtttg aggatctggt ctgcgcaatg atgttgcaca ttcgccagca
      601 ggcgcaggcg cagctgccag aagcgattac tcaggcggtg attggtcgcc cgatcaactt
      661 ccaggggctg ggcggcgatg aagcaaacgc ccaggcgcaa gagattctgg aacgcgcggc
      721 gaagcgtgcc ggattcaggg acgtggtatt ccagtacgag ccgatcgcgg ctgggctgga
      781 ttacgaagcc accttgcagg aagaaaaacg ggtgctggtg gtggatatcg gcggtggtac
      841 gactgactgt tcattgctgc tgatggggcc gcagtggcgt tcgcgtctcg atcgtgaagc
      901 cagtctgctg ggtcacagtg gttgtcgtat tggcggtaac gatctggata tcgccctggc
      961 gtttaaaaac ctgatgccac tgctgggtat gggtggcgaa accgaaaaag gcatcgccct
     1021 gccgatcctg ccgtggtgga atgcggttgc catcaacgac gtacctgcgc agagtgattt
     1081 ctacagtagt gccaacggtc gtctgcttaa cgatctggta cgcgatgccc gcgaaccgga
     1141 aaaagtggcc ctgttacaga aagtctggcg tcagcgttta agctatcgcc tggtgcgtag
     1201 cgcagaagag tgcaaaattg ctctttcaag cgtagcggaa acccgcgcct cactgccgtt
     1261 tatcagcaat gaactggcta cgctgattag ccagcgaggg ctggaaagcg ccctcagcca
     1321 gccactggcg cggattctgg aacaggtgca actggccctg gataacgccc aggaaaaacc
     1381 ggacgttatc tacctgaccg gcggtagcgc ccgttctccg cagattaaaa aagcgctggc
     1441 agaacagttg ccgggcattc cgattgcagg cggcgatgac tttggctccg tcaccgccgg
     1501 gctggcacgc tgggcggaag tggtgtttcg ttaa
//