BBa_J119092
1
BBa_J119092
pLacI-HSP (from Shigella sonnei)
2012-03-27T11:00:00Z
2015-08-31T04:08:27Z
MWSU
This part contains the pLacI promoter and a putative heat shock protein from Shigella sonnei. The gene is the yegD chaperone.
false
false
_613_
0
10415
9
Not in stock
false
none
false
Michel Conn
component2171514
1
BBa_J119091
component2171512
1
BBa_S03511
annotation2171514
1
BBa_J119091
range2171514
1
230
1534
annotation2171512
1
BBa_S03511
range2171512
1
1
223
BBa_S03511
1
BBa_S03511
pLac : RBS
2006-07-20T11:00:00Z
2015-05-08T01:14:24Z
false
true
_61_
0
919
61
It's complicated
false
false
Davidson and Missouri Western
component1886267
1
BBa_R0010
component1886275
1
BBa_B0030
annotation1886275
1
BBa_B0030
range1886275
1
209
223
annotation1886267
1
BBa_R0010
range1886267
1
1
200
BBa_J119091
1
BBa_J119091
HSP (from Shigella sonnei)
2012-03-27T11:00:00Z
2015-08-31T04:08:27Z
The part is from Shigella sonnei ATCC 25931
This part contains a putative heat shock protein from Shigella sonnei. This is the yegD chaperone gene
false
false
_613_
0
10415
9
Not in stock
false
none
false
Michel Conn
annotation2171501
1
Heat Shock Protein (yegD Chaperone
range2171501
1
1
1305
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961227
1
start
range1961227
1
173
173
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_J119091_sequence
1
atgcgtgacggtaaaccgcaattactgaaaatggaaaacgacagcacgctgctgccttcaatgctttgcgcgccaacgcgtgaatcggtaagcgaatggctgtaccgccatcatgatgttccggcagacgacgatgaaacgcaggcgctgttacgtcgggcgattcgttataaccgcgaagaagatatcgatgttacggcgaaaagcgtgcagttcggtctttcctcactggcacagtacattgatgatccagaagaagtgtggtttgtgaaatcaccaaaatcgttcctcggtgccagcggcttaaaaccgcagcaggtagcgctgtttgaggatctggtctgcgcaatgatgttgcacattcgccagcaggcgcaggcgcagctgccagaagcgattactcaggcggtgattggtcgcccgatcaacttccaggggctgggcggcgatgaagcaaacgcccaggcgcaagagattctggaacgcgcggcgaagcgtgccggattcagggacgtggtattccagtacgagccgatcgcggctgggctggattacgaagccaccttgcaggaagaaaaacgggtgctggtggtggatatcggcggtggtacgactgactgttcattgctgctgatggggccgcagtggcgttcgcgtctcgatcgtgaagccagtctgctgggtcacagtggttgtcgtattggcggtaacgatctggatatcgccctggcgtttaaaaacctgatgccactgctgggtatgggtggcgaaaccgaaaaaggcatcgccctgccgatcctgccgtggtggaatgcggttgccatcaacgacgtacctgcgcagagtgatttctacagtagtgccaacggtcgtctgcttaacgatctggtacgcgatgcccgcgaaccggaaaaagtggccctgttacagaaagtctggcgtcagcgtttaagctatcgcctggtgcgtagcgcagaagagtgcaaaattgctctttcaagcgtagcggaaacccgcgcctcactgccgtttatcagcaatgaactggctacgctgattagccagcgagggctggaaagcgccctcagccagccactggcgcggattctggaacaggtgcaactggccctggataacgcccaggaaaaaccggacgttatctacctgaccggcggtagcgcccgttctccgcagattaaaaaagcgctggcagaacagttgccgggcattccgattgcaggcggcgatgactttggctccgtcaccgccgggctggcacgctgggcggaagtggtgtttcgttaa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_S03511_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaa
BBa_J119092_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgcgtgacggtaaaccgcaattactgaaaatggaaaacgacagcacgctgctgccttcaatgctttgcgcgccaacgcgtgaatcggtaagcgaatggctgtaccgccatcatgatgttccggcagacgacgatgaaacgcaggcgctgttacgtcgggcgattcgttataaccgcgaagaagatatcgatgttacggcgaaaagcgtgcagttcggtctttcctcactggcacagtacattgatgatccagaagaagtgtggtttgtgaaatcaccaaaatcgttcctcggtgccagcggcttaaaaccgcagcaggtagcgctgtttgaggatctggtctgcgcaatgatgttgcacattcgccagcaggcgcaggcgcagctgccagaagcgattactcaggcggtgattggtcgcccgatcaacttccaggggctgggcggcgatgaagcaaacgcccaggcgcaagagattctggaacgcgcggcgaagcgtgccggattcagggacgtggtattccagtacgagccgatcgcggctgggctggattacgaagccaccttgcaggaagaaaaacgggtgctggtggtggatatcggcggtggtacgactgactgttcattgctgctgatggggccgcagtggcgttcgcgtctcgatcgtgaagccagtctgctgggtcacagtggttgtcgtattggcggtaacgatctggatatcgccctggcgtttaaaaacctgatgccactgctgggtatgggtggcgaaaccgaaaaaggcatcgccctgccgatcctgccgtggtggaatgcggttgccatcaacgacgtacctgcgcagagtgatttctacagtagtgccaacggtcgtctgcttaacgatctggtacgcgatgcccgcgaaccggaaaaagtggccctgttacagaaagtctggcgtcagcgtttaagctatcgcctggtgcgtagcgcagaagagtgcaaaattgctctttcaagcgtagcggaaacccgcgcctcactgccgtttatcagcaatgaactggctacgctgattagccagcgagggctggaaagcgccctcagccagccactggcgcggattctggaacaggtgcaactggccctggataacgcccaggaaaaaccggacgttatctacctgaccggcggtagcgcccgttctccgcagattaaaaaagcgctggcagaacagttgccgggcattccgattgcaggcggcgatgactttggctccgtcaccgccgggctggcacgctgggcggaagtggtgtttcgttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z