LOCUS       BBa_J119367              968 bp    DNA     linear       01-Jun-2024
DEFINITION  tClone: Device for Testing Transciptional Terminators and 
            Riboswitches via Golden Gate Assembly
ACCESSION   BBa_J119367
VERSION     BBa_J119367.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=P5 Promoter
     misc_feature    42..47
                     /label=BsaI Left
     stem_loop       48..104
                     /label=L3SP22 Txn Terminator
     CDS             112..831
                     /label=GFP
     RBS             838..852
                     /label=B0030 RBS rev
     promoter        860..905
                     /label=P2 Promoter rev
     stem_loop       906..957
                     /label=LS1P53 Txn Terminator
     misc_feature    958..963
                     /label=BsaI Right
ORIGIN
        1 ttgacaatta atcatccggc tcgtaattta tgtggacgac tgagaccctc ggtaccaaat
       61 tccagaaaag aggccgcgaa agcggccttt tttcgttttg gtccactaga gttattattt
      121 gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat
      181 gtgatctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt
      241 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
      301 ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga ttccattctt
      361 ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc
      421 aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt
      481 atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat
      541 agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat gctgtttcat
      601 atgatctggg tatctcgcaa agcattgaac accataaccg aaagtagtga caagtgttgg
      661 ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt
      721 tgcatcacct tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa
      781 ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttacgca tctagtattt
      841 ctcctcttta attactagat ccacacatta taggtacaaa aagatgcgaa gtcaatactc
      901 tttttccaat tattgaaggc ctcccaaatc ggggggcctt ttttattgat aacaaaaggt
      961 ctctgcgg
//