BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_J120013
1
BBa_J120013
Sox10 binding site for Mitf promoter
2012-04-02T11:00:00Z
2015-08-31T04:08:29Z
H. sapiens
A short, twenty base pair DNA sequence which functions as a binding site for the Sox10 transcription factor of the Mitf promoter. When Sox10 is bound to this region, it induces production of the sequences encoded after it.
false
false
_617_
0
7766
9
Not in stock
false
N/A
false
Brett Fuller, Austin Jones, Jeremy Gerbig, Sindhu Adhikari, Coby Turner
BBa_J120014
1
BBa_J120014
Binding sites for Pax3 and Sox10 with GFP selectable marker and double terminator
2012-04-02T11:00:00Z
2015-08-31T04:08:29Z
Composite
Contains two short binding sites (BBa_J120012 and BBa_J120013) followed by a GFP selectable marker (BBa_K259006). When these binding sites have their respective transcription factors bound to them, they will induce the production of GFP, which is a visually detectable marker.
false
false
_617_
0
7766
9
Not in stock
false
N/A
false
Brett Fuller, Austin Jones, Jeremy Gerbig, Sindhu Adhikari, Coby Turner
component2171804
1
BBa_J120013
component2171813
1
BBa_K259006
component2171803
1
BBa_J120012
annotation2171813
1
BBa_K259006
range2171813
1
52
874
annotation2171803
1
BBa_J120012
range2171803
1
1
16
annotation2171804
1
BBa_J120013
range2171804
1
25
45
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J120012
1
BBa_J120012
Pax3 binding site from Mitf promoter
2012-04-02T11:00:00Z
2015-08-31T04:08:29Z
H. sapiens
Short, sixteen base pair DNA sequence that the Pax3 transcription factor binds to. When bound to this region, this promotes the transcription of products encoded after it.
false
false
_617_
0
7766
9
Not in stock
false
N/A
false
Brett Fuller, Austin Jones, Jeremy Gerbig, Sindhu Adhikari, Coby Turner
BBa_K259006
1
BBa_K259006
GFP-Terminator
2009-09-08T11:00:00Z
2015-05-08T01:11:42Z
GFP originates from jellyfish Aequeora victoria. For more details visit the [http://www.expasy.ch/uniprot/P42212 SwissProt entry].
The double terminator was designed by Reshma Shetty. For more details visis the [http://partsregistry.org/Part:BBa_B0014 original part page].
==Part Description==
This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.
Green Fluorescent protein (originally from part [http://partsregistry.org/Part:BBa_E0040 BBa_E0040]) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene.
This part is directly fused to a terminator (originally from part [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]) so it is ideal to be fused at the end of your construct.
==Table of Statistics==
{| style="color:white; background-color:#0066CC;" cellpadding="20" cellspacing="0" border="2"
!Chassis
!Monomer/Multimer
!Aminoacid Length/Weight
!Localisation
!Structure
!Excitation wavelengths
!Emmision wavelengths
|-
|Prokaryotic and Eukaryotic
|Bipartite
|274 aminoacids
|
|beta-barrel(for GFP) hairpins for Terminator
|501nm
|511nm
|}
==References==
<biblio>
#Valdivia pmid=8707053
</biblio>
false
false
_357_
0
5318
9
It's complicated
false
No additional design considerations. The parts were fused together following RFC10 assembly.
false
Petros Mina
component2020529
1
BBa_E0040
component2020530
1
BBa_B0012
component2020534
1
BBa_B0011
annotation2020529
1
BBa_E0040
range2020529
1
1
720
annotation2020534
1
BBa_B0011
range2020534
1
778
823
annotation2020530
1
BBa_B0012
range2020530
1
729
769
BBa_K259006_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_J120014_sequence
1
attaatactactggactactagagtgaaagagaaagaccattgtctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_J120012_sequence
1
attaatactactggac
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_J120013_sequence
1
tgaaagagaaagaccattgtc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z