LOCUS       BBa_J13001              2605 bp    DNA     linear       20-May-2024
DEFINITION  LuxR generator.  POPS generate cI(LVA)  and LuxI(LVA)
ACCESSION   BBa_J13001
VERSION     BBa_J13001.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..729
                     /label=cI lambda
     misc_feature    730..762
                     /label=LVA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             19..768
                     /label=BBa_C0051
     misc_feature    806..809
                     /label=conserved
     RBS             802..813
                     /label=BBa_B0034
     misc_feature    1399..1430
                     /label=LVA
     CDS             820..1398
                     /label=luxI
     misc_feature    820..1437
                     /label=BBa_C0061
     misc_feature    1438..1462
                     /label=Help:Barcodes
     CDS             820..1437
                     /label=BBa_C0061
     stem_loop       1482..1525
                     /label=stem_loop
     misc_feature    1471..1550
                     /label=BBa_B0010
     terminator      1471..1550
                     /label=BBa_B0010
     stem_loop       1566..1585
                     /label=T7 TE
     misc_feature    1592..1592
                     /label=stop
     polyA_site      1586..1599
                     /label=polya
     misc_feature    1559..1599
                     /label=BBa_B0012
     terminator      1559..1599
                     /label=BBa_B0012
     misc_feature    1608..1626
                     /label=TetR 1
     misc_feature    1608..1661
                     /label=BBa_R0040
     promoter        1627..1632
                     /label=-35
     misc_feature    1633..1651
                     /label=TetR 2
     promoter        1650..1655
                     /label=-10
     promoter        1608..1661
                     /label=BBa_R0040
     misc_feature    1674..1677
                     /label=conserved
     RBS             1670..1681
                     /label=BBa_B0034
     misc_feature    1688..1689
                     /label=prefix
     misc_feature    1861..1861
                     /label=T
     misc_feature    2179..2179
                     /label=A
     CDS             1688..2437
                     /label=luxR
     misc_feature    1688..2443
                     /label=BBa_C0062
     misc_feature    2444..2468
                     /label=Help:Barcodes
     CDS             1688..2443
                     /label=BBa_C0062
     stem_loop       2488..2531
                     /label=stem_loop
     misc_feature    2477..2556
                     /label=BBa_B0010
     terminator      2477..2556
                     /label=BBa_B0010
     stem_loop       2572..2591
                     /label=T7 TE
     misc_feature    2598..2598
                     /label=stop
     polyA_site      2592..2605
                     /label=polya
     misc_feature    2565..2605
                     /label=BBa_B0012
     terminator      2565..2605
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc
      781 tagtgtagat cgctactaga gaaagaggag aaatactaga tgactataat gataaaaaaa
      841 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa
      901 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag
      961 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga
     1021 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg
     1081 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta
     1141 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa
     1201 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca
     1261 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa
     1321 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt
     1381 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc
     1441 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt
     1501 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1561 acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga
     1621 tagagattga catccctatc agtgatagag atactgagca ctactagaga aagaggagaa
     1681 atactagatg aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc
     1741 ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg
     1801 tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat
     1861 cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata
     1921 tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga atatatttga
     1981 aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct
     2041 tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt
     2101 tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat
     2161 accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa
     2221 atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa
     2281 aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt
     2341 aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat
     2401 tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg
     2461 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2521 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2581 tcgggtgggc ctttctgcgt ttata
//