LOCUS       BBa_J13009               993 bp    DNA     linear       17-May-2024
DEFINITION  LacI repressed pcyA from synechosystis
ACCESSION   BBa_J13009
VERSION     BBa_J13009.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..831
                     /label=PcyA
     misc_feature    832..856
                     /label=Help:Barcodes
     CDS             82..831
                     /label=BBa_I15009
     stem_loop       876..919
                     /label=stem_loop
     misc_feature    865..944
                     /label=BBa_B0010
     terminator      865..944
                     /label=BBa_B0010
     stem_loop       960..979
                     /label=T7 TE
     misc_feature    986..986
                     /label=stop
     polyA_site      980..993
                     /label=polya
     misc_feature    953..993
                     /label=BBa_B0012
     terminator      953..993
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggccgtc actgatttaa gtttgaccaa ttcttccctg
      121 atgcctacgt tgaacccgat gattcaacag ttggccctgg cgatcgccgc tagttggcaa
      181 agtttacccc tcaagcccta tcaattgccg gaggatttgg gctacgtaga aggccgcctg
      241 gaaggggaaa agttagtgat tgaaaatcgg tgctaccaaa cgccccagtt tcgcaaaatg
      301 catttggagt tggccaaggt gggcaaaggg ttggatattc tccactgtgt aatgtttcct
      361 gagcctttat acggtctacc tttgtttggc tgtgacattg tggccggccc cggtggagta
      421 agtgcggcta ttgcggatct atcccccacc caaagcgatc gccaattgcc cgcagcgtac
      481 caaaaatcat tggcagagct aggccagcca gaatttgagc aacaacggga attgcccccc
      541 tggggagaaa tattttctga atattgttta ttcatccgtc ccagcaatgt cactgaagaa
      601 gaaagatttg tacaaagggt agtggacttt ttgcaaattc attgtcacca atccatcgtt
      661 gccgaaccct tgtctgaagc tcaaactttg gagcaccgtc aggggcaaat tcattactgc
      721 caacaacaac agaaaaatga taaaacccgt cgggtactgg aaaaagcttt tggggaagct
      781 tgggcggaac ggtatatgag ccaagtctta tttgatgtta tccaataata actctgatag
      841 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      961 gctcaccttc gggtgggcct ttctgcgttt ata
//