LOCUS       BBa_J13010              2481 bp    DNA     linear       17-May-2024
DEFINITION  LacI repressed Cph8
ACCESSION   BBa_J13010
VERSION     BBa_J13010.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..2319
                     /label=Cph8
     misc_feature    2320..2344
                     /label=Help:Barcodes
     CDS             82..2319
                     /label=BBa_I15010
     stem_loop       2364..2407
                     /label=stem_loop
     misc_feature    2353..2432
                     /label=BBa_B0010
     terminator      2353..2432
                     /label=BBa_B0010
     stem_loop       2448..2467
                     /label=T7 TE
     misc_feature    2474..2474
                     /label=stop
     polyA_site      2468..2481
                     /label=polya
     misc_feature    2441..2481
                     /label=BBa_B0012
     terminator      2441..2481
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggccacc accgtacaac tcagcgacca atccctccgt
      121 cagctagaaa ccctcgccat ccacaccgcc cacctgattc agccccacgg tttagtggtg
      181 gtcctgcaag aaccagacct caccatcagc caaattagcg ccaactgcac cggcatttta
      241 gggcgatcgc cagaggattt gttgggcaga accctagggg aagtgtttga tagctttcag
      301 attgatccca tccagagtcg cctaacggcc ggacaaatca gcagcctcaa ccccagtaaa
      361 ctttgggcgc gggtcatggg ggacgacttt gtcatttttg acggggtttt tcatcgcaac
      421 agtgacggtt tattggtatg tgaactcgag ccagcctaca cttccgataa tctgcccttc
      481 ctcggttttt atcacatggc caacgctgcc ctgaatcggt tgcgccaaca agctaatcta
      541 cgggatttct acgatgttat tgtcgaagaa gtccgccgta tgactggctt tgaccgggtg
      601 atgctatacc gctttgatga aaataaccac ggtgatgtca ttgccgaaga taaacgggat
      661 gatatggaac cctatttggg cctgcactat cccgaatcgg atattcccca acccgcccgt
      721 cggctattta tccacaaccc cattcgagta attcccgatg tttatggtgt ggcggtgccc
      781 ctgaccccag cggttaaccc cagcaccaac cgagcggtgg atttaacaga atccattctg
      841 cgcagtgcgt accattgcca cttgacctat ctgaaaaata tgggggtagg agcgtcttta
      901 accatttccc taattaagga cggccatctc tgggggctca ttgcctgcca ccatcaaacc
      961 cccaaagtaa ttccctttga actgcgtaaa gcctgcgaat tttttggtcg ggtggtgttt
     1021 agcaacattt ccgcccagga agatacggaa accttcgatt accgggtgca gctggcggag
     1081 catgaagcgg ttttattgga caaaatgacc acggcggcgg attttgtcga aggattaact
     1141 aatcatcccg atcgcctgtt gggattaacg ggctcccagg gggcggccat ttgctttggg
     1201 gaaaaattga ttttagtagg ggaaaccccg gacgagaaag cagtgcaata tttactgcaa
     1261 tggttggaga atcgggaagt gcaagacgtt ttcttcacct cttccctctc acaaatttat
     1321 cctgatgcag tgaattttaa atccgtggcc agtggcttat tggccattcc cattgcccgt
     1381 cacaactttt tgctctggtt tcgccctgaa gtgttgcaaa cggttaattg gggcggtgac
     1441 ccaaatcatg cttacgaagc tacccaggaa gacggtaaaa tcgagctcca tccccgccaa
     1501 tcctttgacc tctggaaaga aattgtccga ctccaatctt tgccctggca atcggtggaa
     1561 atccaaagtg ccctggccct gaaaaaggcg atcgtcaacc tcattttgcg ccaggcagaa
     1621 gaattgcata tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg
     1681 ggggtaagtc acgacttgcg cacgccgctg acgcgtattc gcctggcgac tgagatgatg
     1741 agcgagcagg atggctatct ggcagaatcg atcaataaag atatcgaaga gtgcaacgcc
     1801 atcattgagc agtttatcga ctacctgcgc accgggcagg agatgccgat ggaaatggcg
     1861 gatcttaatg cagtactcgg tgaggtgatt gctgccgaaa gtggctatga gcgggaaatt
     1921 gaaaccgcgc tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc
     1981 gcggtggcga atatggtggt caacgccgcc cgttatggca atggctgggt caaagtcagc
     2041 agcggaacgg agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg
     2101 ccggaacaac gtaagcacct gttccagccg tttgtccgcg gcgacagtgc gcgcaccatt
     2161 agcggcacgg gattagggct ggcaattgtg cagcgtatcg tggataacca taacgggatg
     2221 ctggagcttg gcaccagcga gcggggcggg ctttccattc gcgcctggct gccagtgccg
     2281 gtaacgcggg cgcagggcat gacaaaagaa gggtaataac tctgatagtg ctagtgtaga
     2341 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2401 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2461 gtgggccttt ctgcgtttat a
//