LOCUS       BBa_J13013               968 bp    DNA     linear       15-May-2024
DEFINITION  Tet repressible pcyA
ACCESSION   BBa_J13013
VERSION     BBa_J13013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    807..831
                     /label=Help:Barcodes
     CDS             81..806
                     /label=BBa_I15008
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagtgtca acttagcttc ccagttgcgg gaagggacga
      121 aaaaatccca ctccatggcg gagaacgtcg gctttgtcaa atgcttcctc aagggcgttg
      181 tcgagaaaaa ttcctaccgt aagctggttg gcaatctcta ctttgtctac agtgccatgg
      241 aagaggaaat ggcaaaattt aaggaccatc ccatcctcag ccacatttac ttccccgaac
      301 tcaaccgcaa acaaagccta gagcaagacc tgcaattcta ttacggctcc aactggcggc
      361 aagaagtgaa aatttctgcc gctggccaag cctatgtgga ccgagtccgg caagtggccg
      421 ctacggcccc tgaattgttg gtggcccatt cctacacccg ttacctgggg gatctttccg
      481 gcggtcaaat tctcaagaaa attgcccaaa atgccatgaa tctccacgat ggtggcacag
      541 ctttctatga atttgccgac attgatgacg aaaaggcttt taaaaatacc taccgtcaag
      601 ctatgaatga tctgcccatt gaccaagcca ccgccgaacg gattgtggat gaagccaatg
      661 acgcctttgc catgaacatg aaaatgttca acgaacttga aggcaacctg atcaaggcga
      721 tcggcattat ggtgttcaac agcctcaccc gtcgccgcag tcaaggcagc accgaagttg
      781 gcctcgccac ctccgaaggc taataacgct gatagtgcta gtgtagatcg ctactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttata
//