BBa_R0065
1
cI+luxR
Promoter (lambda cI and luxR regulated -- hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
Released HQ 2013
cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. </P> <P> </P> <table width="75%" border="1"> <tr> <td><strong>LuxI</strong></td> <td><strong>cI</strong></td> <td><strong>activity of promoter</strong></td> </tr> <tr> <td>+</td> <td>+</td> <td>zero</td> </tr> <tr> <td>+</td> <td>-</td> <td>maximum</td> </tr> <tr> <td>-</td> <td>+</td> <td>zero</td> </tr> <tr> <td>-</td> <td>-</td> <td>leaky (no quantitative information)</td> </tr> </table> <P> </P>
false
false
_1_
0
24
7
In stock
false
<P> <P>This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation1986780
1
OR1 cI
range1986780
1
81
97
annotation1986779
1
-35
range1986779
1
71
76
annotation1986777
1
OR2 cI
range1986777
1
57
73
annotation1986774
1
BBa_R0065
range1986774
1
1
97
annotation1986781
1
-10
range1986781
1
94
97
annotation1986778
1
lux p(R) start
range1986778
1
58
58
annotation1986775
1
Lux Box
range1986775
1
6
25
annotation1986776
1
-10
range1986776
1
47
52
BBa_J13023
1
BBa_J13023
3OC6HSL+LuxR dependent POPS/RIPS generator
2005-06-28T11:00:00Z
2015-08-31T04:08:29Z
Released HQ 2013
produces pops and rips in the presence of luxR/3OC6HSL and the absence of lambda cI.
false
true
_37_
0
88
37
In stock
false
false
Jeff Tabor
component1556583
1
BBa_B0034
component1556571
1
BBa_R0065
annotation1556571
1
BBa_R0065
range1556571
1
1
97
annotation1556583
1
BBa_B0034
range1556583
1
106
117
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J13023_sequence
1
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaa
BBa_R0065_sequence
1
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z