LOCUS       BBa_J13030              1946 bp    DNA     linear       11-May-2024
DEFINITION  AI-1 amplifier
ACCESSION   BBa_J13030
VERSION     BBa_J13030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..151
                     /label=R0079
     misc_feature    152..168
                     /label=CI lambda O1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..168
                     /label=BBa_I14016
     misc_feature    181..184
                     /label=conserved
     RBS             177..188
                     /label=BBa_B0034
     CDS             195..797
                     /label=LasI
     misc_feature    798..830
                     /label=LVA
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             195..836
                     /label=BBa_C0078
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     misc_feature    1007..1025
                     /label=TetR 1
     misc_feature    1007..1060
                     /label=BBa_R0040
     promoter        1026..1031
                     /label=-35
     misc_feature    1032..1050
                     /label=TetR 2
     promoter        1049..1054
                     /label=-10
     promoter        1007..1060
                     /label=BBa_R0040
     misc_feature    1073..1076
                     /label=conserved
     RBS             1069..1080
                     /label=BBa_B0034
     CDS             1087..1809
                     /label=BBa_C0179
     stem_loop       1829..1872
                     /label=stem_loop
     misc_feature    1818..1897
                     /label=BBa_B0010
     terminator      1818..1897
                     /label=BBa_B0010
     stem_loop       1913..1932
                     /label=T7 TE
     misc_feature    1939..1939
                     /label=stop
     polyA_site      1933..1946
                     /label=polya
     misc_feature    1906..1946
                     /label=BBa_B0012
     terminator      1906..1946
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca ctacctctgg cggtgatata ctagagaaag
      181 aggagaaata ctagatgatc gttcagatcg gtcgtcgtga agagttcgac aaaaaactgc
      241 tgggtgaaat gcacaaactg cgtgctcagg ttttcaaaga acgtaaaggt tgggacgttt
      301 ccgttatcga cgaaatggaa atcgacggtt acgacgctct gtccccgtac tacatgctga
      361 tccaggaaga caccccggaa gctcaggttt tcggttgctg gcgtatcttc gacaccaccg
      421 gtccgtacat gctgaaaaac accttcccgg aactgctgca cggtaaagaa gctccgtgct
      481 ccccgcacat ctgggaactg tcccgtttcg ctatcaactc cggtcagaaa ggttccctgg
      541 gtttctccga ctgcaccctg gaagctatgc gtgctctggc tcgttactcc ttgcagaacg
      601 acatccagac cctggttacc gttaccaccg ttggtgttga aaaaatgatg atccgtgctg
      661 gtctggacgt ttcccgtttc ggtccgcacc tgaaaatcgg tatcgaacgt gctgttgctc
      721 tgcgtatcga actgaacgct aaaacccaga tcgctctgta cggtggtgtt ctggttgaac
      781 agcgtctggc tgtttccgct gctaacgacg aaaactacgc tctggttgct taataactct
      841 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat
     1021 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa
     1081 tactagatgg ccttggttga cggttttctt gagctggaac gctcaagtgg aaaattggag
     1141 tggagcgcca tcctccagaa gatggcgagc gaccttggat tctcgaagat cctgttcggc
     1201 ctgttgccta aggacagcca ggactacgag aacgccttca tcgtcggcaa ctacccggcc
     1261 gcctggcgcg agcattacga ccgggctggc tacgcgcggg tcgacccgac ggtcagtcac
     1321 tgtacccaga gcgtactgcc gattttctgg gaaccgtcca tctaccagac gcgaaagcag
     1381 cacgagttct tcgaggaagc ctcggccgcc ggcctggtgt atgggctgac catgccgctg
     1441 catggtgctc gcggcgaact cggcgcgctg agcctcagcg tggaagcgga aaaccgggcc
     1501 gaggccaacc gtttcataga gtcggtcctg ccgaccctgt ggatgctcaa ggactacgca
     1561 ctgcaaagcg gtgccggact ggccttcgaa catccggtca gcaaaccggt ggttctgacc
     1621 agccgggaga aggaagtgtt gcagtggtgc gccatcggca agaccagttg ggagatatcg
     1681 gttatctgca actgctcgga agccaatgtg aacttccata tgggaaatat tcggcggaag
     1741 ttcggtgtga cctcccgccg cgtagcggcc attatggccg ttaatttggg tcttattact
     1801 ctctaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1921 ttcgggtggg cctttctgcg tttata
//