LOCUS BBa_J13031 3863 bp DNA linear 10-May-2024 DEFINITION 3OC6HSL and AI-1 amplifier ACCESSION BBa_J13031 VERSION BBa_J13031.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 misc_feature 703..734 /label=LVA CDS 124..702 /label=luxI misc_feature 124..741 /label=BBa_C0061 misc_feature 742..766 /label=Help:Barcodes CDS 124..741 /label=BBa_C0061 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 misc_feature 912..930 /label=TetR 1 misc_feature 912..965 /label=BBa_R0040 promoter 931..936 /label=-35 misc_feature 937..955 /label=TetR 2 promoter 954..959 /label=-10 promoter 912..965 /label=BBa_R0040 misc_feature 978..981 /label=conserved RBS 974..985 /label=BBa_B0034 misc_feature 992..993 /label=prefix misc_feature 1165..1165 /label=T misc_feature 1483..1483 /label=A CDS 992..1741 /label=luxR misc_feature 992..1747 /label=BBa_C0062 misc_feature 1748..1772 /label=Help:Barcodes CDS 992..1747 /label=BBa_C0062 stem_loop 1792..1835 /label=stem_loop misc_feature 1781..1860 /label=BBa_B0010 terminator 1781..1860 /label=BBa_B0010 stem_loop 1876..1895 /label=T7 TE misc_feature 1902..1902 /label=stop polyA_site 1896..1909 /label=polya misc_feature 1869..1909 /label=BBa_B0012 terminator 1869..1909 /label=BBa_B0012 misc_feature 1918..2068 /label=R0079 misc_feature 2069..2085 /label=CI lambda O1 promoter 2034..2039 /label=-35 promoter 2057..2062 /label=-10 promoter 1918..2085 /label=BBa_I14016 misc_feature 2098..2101 /label=conserved RBS 2094..2105 /label=BBa_B0034 CDS 2112..2714 /label=LasI misc_feature 2715..2747 /label=LVA misc_feature 2754..2778 /label=Help:Barcodes CDS 2112..2753 /label=BBa_C0078 stem_loop 2798..2841 /label=stem_loop misc_feature 2787..2866 /label=BBa_B0010 terminator 2787..2866 /label=BBa_B0010 stem_loop 2882..2901 /label=T7 TE misc_feature 2908..2908 /label=stop polyA_site 2902..2915 /label=polya misc_feature 2875..2915 /label=BBa_B0012 terminator 2875..2915 /label=BBa_B0012 misc_feature 2924..2942 /label=TetR 1 misc_feature 2924..2977 /label=BBa_R0040 promoter 2943..2948 /label=-35 misc_feature 2949..2967 /label=TetR 2 promoter 2966..2971 /label=-10 promoter 2924..2977 /label=BBa_R0040 misc_feature 2990..2993 /label=conserved RBS 2986..2997 /label=BBa_B0034 CDS 3004..3726 /label=BBa_C0179 stem_loop 3746..3789 /label=stem_loop misc_feature 3735..3814 /label=BBa_B0010 terminator 3735..3814 /label=BBa_B0010 stem_loop 3830..3849 /label=T7 TE misc_feature 3856..3856 /label=stop polyA_site 3850..3863 /label=polya misc_feature 3823..3863 /label=BBa_B0012 terminator 3823..3863 /label=BBa_B0012 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa 181 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta 241 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt 301 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg 361 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata 421 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt 481 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca 541 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct 601 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg 661 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 961 agcactacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata 1021 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc 1081 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc 1141 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta 1201 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc 1261 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat 1321 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa 1381 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt 1441 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg 1501 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg 1561 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag 1621 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg 1681 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa 1741 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga 1801 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1861 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagaggcc 1921 cctcgctgag cgcgtcccgg agctgggggc aacctagctg ccacctgctt ttctgctagc 1981 tattccagcg aaaacataca gatttccggc gaaatcaagg ctacctgcca gttctggcag 2041 gtttggccgc gggttctttt tggtacacta cctctggcgg tgatatacta gagaaagagg 2101 agaaatacta gatgatcgtt cagatcggtc gtcgtgaaga gttcgacaaa aaactgctgg 2161 gtgaaatgca caaactgcgt gctcaggttt tcaaagaacg taaaggttgg gacgtttccg 2221 ttatcgacga aatggaaatc gacggttacg acgctctgtc cccgtactac atgctgatcc 2281 aggaagacac cccggaagct caggttttcg gttgctggcg tatcttcgac accaccggtc 2341 cgtacatgct gaaaaacacc ttcccggaac tgctgcacgg taaagaagct ccgtgctccc 2401 cgcacatctg ggaactgtcc cgtttcgcta tcaactccgg tcagaaaggt tccctgggtt 2461 tctccgactg caccctggaa gctatgcgtg ctctggctcg ttactccttg cagaacgaca 2521 tccagaccct ggttaccgtt accaccgttg gtgttgaaaa aatgatgatc cgtgctggtc 2581 tggacgtttc ccgtttcggt ccgcacctga aaatcggtat cgaacgtgct gttgctctgc 2641 gtatcgaact gaacgctaaa acccagatcg ctctgtacgg tggtgttctg gttgaacagc 2701 gtctggctgt ttccgctgct aacgacgaaa actacgctct ggttgcttaa taactctgat 2761 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2821 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2881 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga 2941 gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac 3001 tagatggcct tggttgacgg ttttcttgag ctggaacgct caagtggaaa attggagtgg 3061 agcgccatcc tccagaagat ggcgagcgac cttggattct cgaagatcct gttcggcctg 3121 ttgcctaagg acagccagga ctacgagaac gccttcatcg tcggcaacta cccggccgcc 3181 tggcgcgagc attacgaccg ggctggctac gcgcgggtcg acccgacggt cagtcactgt 3241 acccagagcg tactgccgat tttctgggaa ccgtccatct accagacgcg aaagcagcac 3301 gagttcttcg aggaagcctc ggccgccggc ctggtgtatg ggctgaccat gccgctgcat 3361 ggtgctcgcg gcgaactcgg cgcgctgagc ctcagcgtgg aagcggaaaa ccgggccgag 3421 gccaaccgtt tcatagagtc ggtcctgccg accctgtgga tgctcaagga ctacgcactg 3481 caaagcggtg ccggactggc cttcgaacat ccggtcagca aaccggtggt tctgaccagc 3541 cgggagaagg aagtgttgca gtggtgcgcc atcggcaaga ccagttggga gatatcggtt 3601 atctgcaact gctcggaagc caatgtgaac ttccatatgg gaaatattcg gcggaagttc 3661 ggtgtgacct cccgccgcgt agcggccatt atggccgtta atttgggtct tattactctc 3721 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 3781 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 3841 gggtgggcct ttctgcgttt ata //