LOCUS       BBa_J13061              1688 bp    DNA     linear       18-May-2024
DEFINITION  B0033.CFP/B0034.YFP
ACCESSION   BBa_J13061
VERSION     BBa_J13061.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..73
                     /label=RBS-4\Weaker
     RBS             69..72
                     /label=RBS
     misc_feature    63..73
                     /label=BBa_B0033
     RBS             63..73
                     /label=BBa_B0033
     CDS             80..802
                     /label=BBa_E0020
     misc_feature    815..818
                     /label=conserved
     RBS             811..822
                     /label=BBa_B0034
     CDS             829..1551
                     /label=BBa_E0030
     stem_loop       1571..1614
                     /label=stem_loop
     misc_feature    1560..1639
                     /label=BBa_B0010
     terminator      1560..1639
                     /label=BBa_B0010
     stem_loop       1655..1674
                     /label=T7 TE
     misc_feature    1681..1681
                     /label=stop
     polyA_site      1675..1688
                     /label=polya
     misc_feature    1648..1688
                     /label=BBa_B0012
     terminator      1648..1688
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gactactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg
      301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg tctatatcac
      541 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
      601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      781 ggacgagctg tacaagtaat aatactagag aaagaggaga aatactagat ggtgagcaag
      841 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      901 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      961 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1021 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
     1081 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1141 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1201 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1261 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1321 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1381 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     1441 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1501 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc
     1561 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1621 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1681 cgtttata
//