LOCUS       BBa_J13075              1680 bp    DNA     linear       10-May-2024
DEFINITION  Polycistronic mCherry/YFP
ACCESSION   BBa_J13075
VERSION     BBa_J13075.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..791
                     /label=mCherry
     misc_feature    432..432
                     /label=C->T (removing PstI site)
     misc_feature    81..794
                     /label=BBa_J06504
     misc_feature    807..810
                     /label=conserved
     RBS             803..814
                     /label=BBa_B0034
     CDS             821..1543
                     /label=BBa_E0030
     stem_loop       1563..1606
                     /label=stem_loop
     misc_feature    1552..1631
                     /label=BBa_B0010
     terminator      1552..1631
                     /label=BBa_B0010
     stem_loop       1647..1666
                     /label=T7 TE
     misc_feature    1673..1673
                     /label=stop
     polyA_site      1667..1680
                     /label=polya
     misc_feature    1640..1680
                     /label=BBa_B0012
     terminator      1640..1680
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga ggataacatg gccatcatca
      121 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
      181 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      241 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
      301 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
      361 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
      421 aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
      481 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
      541 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
      601 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      661 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
      721 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      781 tgtacaagta ataatactag agaaagagga gaaatactag atggtgagca agggcgagga
      841 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      901 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      961 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     1021 cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc
     1081 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1141 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1201 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1261 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1321 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1381 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     1441 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1501 cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc
     1561 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1621 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//