LOCUS       BBa_J13080              1672 bp    DNA     linear       17-May-2024
DEFINITION  PolycistronicYFP/mRFP
ACCESSION   BBa_J13080
VERSION     BBa_J13080.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..755
                     /label=mrfp1
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             81..761
                     /label=BBa_E1010
     misc_feature    63..786
                     /label=BBa_I13502
     misc_feature    1..786
                     /label=BBa_J13069
     misc_feature    799..802
                     /label=conserved
     RBS             795..806
                     /label=BBa_B0034
     CDS             813..1535
                     /label=BBa_E0030
     stem_loop       1555..1598
                     /label=stem_loop
     misc_feature    1544..1623
                     /label=BBa_B0010
     terminator      1544..1623
                     /label=BBa_B0010
     stem_loop       1639..1658
                     /label=T7 TE
     misc_feature    1665..1665
                     /label=stop
     polyA_site      1659..1672
                     /label=polya
     misc_feature    1632..1672
                     /label=BBa_B0012
     terminator      1632..1672
                     /label=BBa_B0012
     misc_feature    795..1672
                     /label=BBa_E0430
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca
      841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     1021 aatgcttcgc ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc
     1081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1261 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1381 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     1441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1501 tcactctcgg catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa
     1561 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1621 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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