LOCUS       BBa_J13100              1757 bp    DNA     linear       09-May-2024
DEFINITION  pOmpR dependent dual repressor device for I15030 and J13030
ACCESSION   BBa_J13100
VERSION     BBa_J13100.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             138..845
                     /label=cI lambda
     misc_feature    846..878
                     /label=LVA
     misc_feature    885..909
                     /label=Help:Barcodes
     CDS             135..884
                     /label=BBa_C0051
     misc_feature    922..925
                     /label=conserved
     RBS             918..929
                     /label=BBa_B0034
     CDS             939..1555
                     /label=tetR
     misc_feature    1556..1589
                     /label=SsrA
     misc_feature    1596..1620
                     /label=Help:Barcodes
     CDS             936..1595
                     /label=BBa_C0040
     stem_loop       1640..1683
                     /label=stem_loop
     misc_feature    1629..1708
                     /label=BBa_B0010
     terminator      1629..1708
                     /label=BBa_B0010
     stem_loop       1724..1743
                     /label=T7 TE
     misc_feature    1750..1750
                     /label=stop
     polyA_site      1744..1757
                     /label=polya
     misc_feature    1717..1757
                     /label=BBa_B0012
     terminator      1717..1757
                     /label=BBa_B0012
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg
      181 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat
      241 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca
      301 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag
      361 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc
      421 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct
      481 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa
      541 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag
      601 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg
      661 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga
      721 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat
      781 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt
      841 ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt
      901 gtagatcgct actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag
      961 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
     1021 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
     1081 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
     1141 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
     1201 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
     1261 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
     1321 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
     1381 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
     1441 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
     1501 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg
     1561 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
     1621 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1681 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1741 gcctttctgc gtttata
//