LOCUS       BBa_J153003              946 bp    DNA     linear       17-May-2024
DEFINITION  Ptrc1O-driven GFP reporter
ACCESSION   BBa_J153003
VERSION     BBa_J153003.1  
FEATURES             Location/Qualifiers
     promoter        1..6
                     /label=-35 box
     promoter        24..29
                     /label=-10 box
     misc_feature    36..56
                     /label=lac O1
     promoter        1..63
                     /label=BBa_J153001
     misc_feature    76..79
                     /label=conserved
     RBS             72..83
                     /label=BBa_B0034
     CDS             90..809
                     /label=GFP protein
     CDS             90..809
                     /label=BBa_E0040
     stem_loop       829..872
                     /label=stem_loop
     misc_feature    818..897
                     /label=BBa_B0010
     terminator      818..897
                     /label=BBa_B0010
     stem_loop       913..932
                     /label=T7 TE
     misc_feature    939..939
                     /label=stop
     polyA_site      933..946
                     /label=polya
     misc_feature    906..946
                     /label=BBa_B0012
     terminator      906..946
                     /label=BBa_B0012
     misc_feature    72..946
                     /label=BBa_I13504
ORIGIN
        1 ttgacaatta atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acatactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag
      121 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      181 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      241 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
      301 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
      361 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
      421 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
      481 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
      541 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
      601 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      661 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
      721 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
      781 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//