BBa_J15102 1 BBa_J15102 lacZ' coding sequence 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Escherichia coli BS. The sequence was obtained from gi:146575. Released HQ 2013 Escherichia coli lacZ' coding sequence encoding the first 76 amino acids of LacZ (beta-galactosidase). This is sufficient to complement the lacZ-delta-M15 mutation found in commonly used laboratory strains of E. coli, thus allowing blue-white selection. It also can be used to effect a pH drop in the presence of lactose, as in the arsenic biosensor entered by the University of Ediburgh team for iGEM2006. false false _163_ 0 837 163 In stock false Codon 77, TGC, was changed to TAA to truncate the coding sequence. false Chris French annotation1938053 1 end lacZ range1938053 1 229 231 annotation1938052 1 start lacZ' range1938052 1 1 3 annotation1938051 1 lacZ' range1938051 1 1 231 BBa_J15101 1 BBa_J15101 arsR coding sequence, Escherichia coli chromosomal ars repressor 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Escherichia coli K12 genomic sequence, based on X80057 (gi:510824). Escherichia coli chromosomal arsR coding sequence, which encodes the ArsR repressor. This binds to the ars promoter and represses it in the absence of arsenate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056). false false _163_ 0 837 163 Not in stock false No modifications to the published sequence except that a second TAA stop codon was added in accordance with Registry recommendations. false Chris French annotation1938049 1 end arsR range1938049 1 352 354 annotation1938050 1 StuI range1938050 1 260 265 annotation1938048 1 start arsR range1938048 1 1 3 annotation1938047 1 arsR coding sequence range1938047 1 1 354 BBa_J15403 1 BBa_J15403 ars promoter + lacZ' + arsR 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z The ars promoter and arsR are from E. coli K12 (gi: 510824) and lacZ' from E. coli BS (gi: 146575). This the Escherichia coli chromosomal ars promoter, followed by lacZ' encoding the N-terminal 77 amino acids of LacZ (bet galactosidase), followed by arsR encoding the ArsR repressor which represses the promoter in the absence of arsenate or arsenite (see Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056). Its function is to generate LacZ activity in the presence of arsenate or arsenite. Note that this requires a host strain such as E. coli JM109, with the lacZ-delta-M15 mutation, providing the C-terminal part of LacZ. false false _163_ 0 837 163 Not in stock false No issues false Chris French component1938111 1 BBa_J15301 component1938114 1 BBa_J15001 component1938118 1 BBa_J15102 component1938121 1 BBa_J15001 component1938126 1 BBa_J15101 annotation1938121 1 BBa_J15001 range1938121 1 394 403 annotation1938126 1 BBa_J15101 range1938126 1 410 766 annotation1938118 1 BBa_J15102 range1938118 1 152 385 annotation1938111 1 BBa_J15301 range1938111 1 1 127 annotation1938114 1 BBa_J15001 range1938114 1 136 145 BBa_J15001 1 BBa_J15001 strong synthetic E. coli ribosome binding site with SacI site. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Synthetic. This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector. false false _163_ 0 837 163 Not in stock false Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest. false Chris French annotation1938045 1 SacI range1938045 1 1 3 annotation1938046 1 rbs range1938046 1 4 10 BBa_J15301 1 BBa_J15301 Pars promoter from Escherichia coli chromosomal ars operon. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Escherichia coli K12. Sequence obtained from X80057 (gi:510824). Regulatory sequence annotation from Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056. This is the promoter region of the Escherichia coli chromosomal ars promoter. It is repressed by ArsR in the absence of arsenate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056). false false _163_ 0 837 163 Not in stock false No modifications were made to the sequence. There appears to be an unexpected repeat of ACT immediately before the SpeI site. This does not affect the biobrick suffix but means that the suffix is 5 bases downstream of the annotated transcriptional start site rather than 2 as recommended. The reason for this is unclear. false Chris French annotation1938077 1 -10 range1938077 1 111 116 annotation1938074 1 ArsR binding range1938074 1 67 71 annotation1938078 1 +1 range1938078 1 123 123 annotation1938075 1 ArsR binding range1938075 1 81 85 annotation1938076 1 -35 range1938076 1 88 93 BBa_J15102_sequence 1 atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa BBa_J15403_sequence 1 tcctgattcagacctcctttcaaatgaatagccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactactactagagctcaaggaggtactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagctcaaggaggtactagatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaataa BBa_J15001_sequence 1 ctcaaggagg BBa_J15101_sequence 1 atgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaataa BBa_J15301_sequence 1 tcctgattcagacctcctttcaaatgaatagccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z