LOCUS       BBa_J176051             3085 bp    DNA     linear       17-May-2024
DEFINITION  miRNA CFP-reporter 2
ACCESSION   BBa_J176051
VERSION     BBa_J176051.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     misc_feature    523..539
                     /label=Gal4
     misc_feature    542..558
                     /label=Gal4
     misc_feature    561..577
                     /label=Gal4
     misc_feature    580..596
                     /label=Gal4
     misc_feature    599..615
                     /label=Gal4
     misc_feature    523..615
                     /label=BBa_J176019
     misc_feature    1..615
                     /label=BBa_S04643
     misc_feature    616..621
                     /label=BBa_J176041
     RBS             622..639
                     /label=BBa_J176012
     misc_feature    640..645
                     /label=BBa_J176041
     misc_feature    646..1179
                     /label=BBa_J176018
     misc_feature    1180..1185
                     /label=BBa_J176041
     misc_feature    1186..1872
                     /label=BBa_J176022
     misc_feature    1873..1878
                     /label=BBa_J176041
     misc_feature    1879..2565
                     /label=BBa_J176022
     misc_feature    2566..2571
                     /label=BBa_J176041
     misc_feature    2572..2592
                     /label=BBa_J176008
     misc_feature    2593..2598
                     /label=BBa_J176041
     terminator      2599..2611
                     /label=BBa_J176016
     misc_feature    2612..2617
                     /label=BBa_J176041
     misc_feature    2745..2745
                     /label=questionable base call
     misc_feature    2618..3085
                     /label=BBa_J176042
     misc_feature    622..3085
                     /label=BBa_S04704
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga
      541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      601 gagtactgtc ctccgactag acccgccgcc accatggaga ctagagcatc caacttgaac
      661 atttcgagaa agcttaccat atcaacccca tcatgctctt tcgaaaattc aaatagcaca
      721 tccattcctt cgcccgcttc ctcatctcaa agccacactc caatgagaaa catgagctca
      781 ctctctgata acagcgtttt cagccggaat atggaacaat catcaccaat cactccaagt
      841 atgtaccaat ttggtcagca gcagtcaaac agtatatgtg gtagcaccgt tagtgtgaat
      901 agtctggtga atacaaataa caaacaaagg atctacgaac aaatcacggg tcctaacagc
      961 aataacgcaa ccaatgatta tattgatttg ctaaacctaa atgagtctaa caaggaaaac
     1021 caaaatcccg caacggcgca ttacctcaat gggggcccac ccaagacaag cttcattaac
     1081 catggaatgt tcccctcgcc aactgggacc ataaatagcg gtaaatctag cagtgcctca
     1141 tctttaattt cttttggtat gggcaatacc caagtaataa ctagaatggc cctgtccaac
     1201 aagttcatcg gcgacgacat gaagatgacc taccacatgg acggctgcgt gaacggccac
     1261 tacttcaccg tgaagggcga gggcagcggc aagccctacg agggcaccca gacctccacc
     1321 ttcaaggtga ccatggccaa cggcggcccc ctggccttct ccttcgacat cctgtccacc
     1381 gtgttcatgt acggcaaccg ctgcttcacc gcctacccca ccagcatgcc cgactacttc
     1441 aagcaggcct tccccgacgg catgtcctac gagagaacct tcacctacga ggacggcggc
     1501 gtggccaccg ccagctggga gatcagcctg aagggcaact gcttcgagca caagtccacc
     1561 ttccacggcg tgaacttccc cgccgacggc cccgtgatgg ccaagaagac caccggctgg
     1621 gacccctcct tcgagaagat gaccgtgtgc gacggcatct tgaagggcga cgtgaccgcc
     1681 ttcctgatgc tgcaaggcgg cggcaactac agatgccagt tccacacctc ctacaagacc
     1741 aagaagcccg tgaccatgcc ccccaaccac gtggtggagc accgcatcgc cagaaccgac
     1801 ctggacaagg gcggcaacag cgtgcagctg accgagcacg ccgtggccca catcacctcc
     1861 gtggtgccct tcactagaat ggccctgtcc aacaagttca tcggcgacga catgaagatg
     1921 acctaccaca tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc
     1981 ggcaagccct acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc
     2041 cccctggcct tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc
     2101 accgcctacc ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc
     2161 tacgagagaa ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc
     2221 ctgaagggca actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac
     2281 ggccccgtga tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg
     2341 tgcgacggca tcttgaaggg cgacgtgacc gccttcctga tgctgcaagg cggcggcaac
     2401 tacagatgcc agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac
     2461 cacgtggtgg agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag
     2521 ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc ccttcactag acccaagaaa
     2581 aagcgcaagg taactagagc gtaaagctga gactagaaga catgataaga tacattgatg
     2641 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2701 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2761 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     2821 acctctacaa atgtggtatg gctgattatg atcctgcaag cctcgtcgtc ctggccggac
     2881 cacgctatct gtgcaaggtc cccggccccg gacgcgcgct ccatgagcag agcgcccgcc
     2941 gccgaggcga agactcgggc ggcgccctgc ccgtcccacc aggtcaacag gcggtaaccg
     3001 gcctcttcat cgggaatgcg cgcgaccttc agcatcgccg gcatgtcccc ctggcggacg
     3061 ggaagtatcc agctcgacca agctt
//