LOCUS BBa_J176072 3618 bp DNA linear 17-May-2024 DEFINITION EED expression casette 2 ACCESSION BBa_J176072 VERSION BBa_J176072.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 RBS 523..540 /label=BBa_J176012 misc_feature 1..540 /label=BBa_S04645 misc_feature 541..546 /label=BBa_J176041 misc_feature 547..1005 /label=BBa_J176020 misc_feature 1006..1011 /label=BBa_J176041 misc_feature 1012..2412 /label=BBa_J176023 misc_feature 547..2412 /label=BBa_S04680 misc_feature 2413..2418 /label=BBa_J176041 misc_feature 2419..2452 /label=BBa_J176024 misc_feature 1..2452 /label=BBa_J176071 misc_feature 2453..2458 /label=BBa_J176041 misc_feature 2586..2586 /label=questionable base call misc_feature 2459..2926 /label=BBa_J176042 misc_feature 2927..2932 /label=BBa_J176041 misc_feature 2933..3272 /label=BBa_J176021 misc_feature 3273..3278 /label=BBa_J176041 misc_feature 3279..3618 /label=BBa_J176021 misc_feature 2459..3618 /label=BBa_S04679 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacccgccgc caccatggag 541 actagaatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag 601 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt 661 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa 721 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac 781 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta 841 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg 901 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac 961 aaaggtcaaa gacagttgac tgtatcgccg gaatttccgg ggatcactag aatgtccgag 1021 agggaagtgt cgactgcgcc ggcgggaaca gacatgcctg cggccaagaa gcagaagctg 1081 agcagtgacg agaacagcaa tccagacctc tctggagacg agaatgatga cgctgtcagt 1141 atagaaagtg gtacaaacac tgaacgccct gatacaccta caaacacgcc aaatgcacct 1201 ggaaggaaaa gttggggaaa gggaaaatgg aagtcaaaga aatgcaaata ttctttcaaa 1261 tgtgtaaata gtctcaagga agatcataac caaccattgt ttggagttca gtttaactgg 1321 cacagtaaag aaggagatcc attagtgttt gcaactgtag gaagcaacag agttaccttg 1381 tatgaatgtc attcacaagg agaaatccgg ttgttgcaat cttacgtgga tgctgatgct 1441 gatgaaaact tttacacttg tgcatggacc tatgatagca atacgagcca tcctctgctg 1501 gctgtagctg gatcaagagg cataattagg ataataaatc ctataacaat gcagtgtata 1561 aagcactatg ttggccatgg aaatgctatc aatgagctga aattccatcc aagagatcca 1621 aatcttctcc tgtcagtaag taaagatcat gctttacgat tatggaatat ccagacggac 1681 actctggtgg caatatttgg aggcgtagaa gggcacagag atgaagttct aagtgctgat 1741 tatgatcttt tgggtgaaaa aataatgtcc tgtggtatgg atcattctct taaactttgg 1801 aggatcaatt caaagagaat gatgaatgca attaaggaat cttatgatta taatccaaat 1861 aaaactaaca ggccatttat ttctcagaaa atccattttc ctgatttttc taccagagac 1921 atacatagga attatgttga ttgtgtgcga tggttaggcg atttgatact ttctaagagt 1981 ggccgtgcca ttttacattc ccaccagcaa tgtatgagag atccagtgtc tccgaatctt 2041 cgccagcatt tgtcttgtga aaatgccatt gtgtgctgga aacctggcaa gatggaagat 2101 gatatagata aaattaaacc cagtgaatct aatgtgacta ttcttgggcg atttgattac 2161 agccagtgtg acatttggta catgaggttt tctatggatt tctggcaaaa gatgcttgca 2221 ttgggcaatc aagttggcaa actttatgtt tgggatttag aagtagaaga tcctcataaa 2281 gccaaatgta caacactgac tcatcataaa tgtggtgctg ctattcgaca aaccagtttt 2341 agcagggata gcagcattct tatagctgtt tgtgatgatg ccagtatttg gcgctgggat 2401 cgacttcgat tgactagacc caagaaaaag cgcaaggtag cgtaaagctg agactagaag 2461 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 2521 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 2581 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 2641 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctgcaa 2701 gcctcgtcgt cctggccgga ccacgctatc tgtgcaaggt ccccggcccc ggacgcgcgc 2761 tccatgagca gagcgcccgc cgccgaggcg aagactcggg cggcgccctg cccgtcccac 2821 caggtcaaca ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcgcc 2881 ggcatgtccc cctggcggac gggaagtatc cagctcgacc aagcttacta gattaattaa 2941 ctcgagtcct aaagaagtcg gcttctccat cggatcctga tcaggggagc tcacggggac 3001 agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag 3061 cgaccgcccg gggctccgct ccggtccggc gctccccccg catcccgagc cggcagcgtg 3121 cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg 3181 ctgctctttg agcctgcaaa cacctggggg atacggggaa aatgatcaag atcttctaca 3241 ctgggacgca aggattatcc tcgaggcgcg ccactagatt aattaactcg agtcctaaag 3301 aagtcggctt ctccatcgga tcctgatcag gggagctcac ggggacagcc cccccccaaa 3361 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgac cgcccggggc 3421 tccgctccgg tccggcgctc cccccgcatc ccgagccggc agcgtgcggg gacagcccgg 3481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc 3541 tgcaaacacc tgggggatac ggggaaaatg atcaagatct tctacactgg gacgcaagga 3601 ttatcctcga ggcgcgcc //