LOCUS       BBa_J176090             2743 bp    DNA     linear       15-May-2024
DEFINITION  KAH180
ACCESSION   BBa_J176090
VERSION     BBa_J176090.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     RBS             523..540
                     /label=BBa_J176012
     misc_feature    541..546
                     /label=BBa_J176041
     misc_feature    547..1080
                     /label=BBa_J176018
     misc_feature    1081..1086
                     /label=BBa_J176041
     misc_feature    1087..1773
                     /label=BBa_J176022
     misc_feature    1774..1779
                     /label=BBa_J176041
     misc_feature    1780..2466
                     /label=BBa_J176022
     misc_feature    2467..2472
                     /label=BBa_J176041
     misc_feature    2473..2493
                     /label=BBa_J176008
     misc_feature    2494..2499
                     /label=BBa_J176041
     terminator      2500..2512
                     /label=BBa_J176016
     misc_feature    523..2512
                     /label=BBa_S04733
     misc_feature    2513..2518
                     /label=BBa_J176041
     misc_feature    2519..2743
                     /label=BBa_J176009
     misc_feature    523..2743
                     /label=BBa_S04734
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacccgccgc caccatggag
      541 actagagcat ccaacttgaa catttcgaga aagcttacca tatcaacccc atcatgctct
      601 ttcgaaaatt caaatagcac atccattcct tcgcccgctt cctcatctca aagccacact
      661 ccaatgagaa acatgagctc actctctgat aacagcgttt tcagccggaa tatggaacaa
      721 tcatcaccaa tcactccaag tatgtaccaa tttggtcagc agcagtcaaa cagtatatgt
      781 ggtagcaccg ttagtgtgaa tagtctggtg aatacaaata acaaacaaag gatctacgaa
      841 caaatcacgg gtcctaacag caataacgca accaatgatt atattgattt gctaaaccta
      901 aatgagtcta acaaggaaaa ccaaaatccc gcaacggcgc attacctcaa tgggggccca
      961 cccaagacaa gcttcattaa ccatggaatg ttcccctcgc caactgggac cataaatagc
     1021 ggtaaatcta gcagtgcctc atctttaatt tcttttggta tgggcaatac ccaagtaata
     1081 actagaatgg ccctgtccaa caagttcatc ggcgacgaca tgaagatgac ctaccacatg
     1141 gacggctgcg tgaacggcca ctacttcacc gtgaagggcg agggcagcgg caagccctac
     1201 gagggcaccc agacctccac cttcaaggtg accatggcca acggcggccc cctggccttc
     1261 tccttcgaca tcctgtccac cgtgttcatg tacggcaacc gctgcttcac cgcctacccc
     1321 accagcatgc ccgactactt caagcaggcc ttccccgacg gcatgtccta cgagagaacc
     1381 ttcacctacg aggacggcgg cgtggccacc gccagctggg agatcagcct gaagggcaac
     1441 tgcttcgagc acaagtccac cttccacggc gtgaacttcc ccgccgacgg ccccgtgatg
     1501 gccaagaaga ccaccggctg ggacccctcc ttcgagaaga tgaccgtgtg cgacggcatc
     1561 ttgaagggcg acgtgaccgc cttcctgatg ctgcaaggcg gcggcaacta cagatgccag
     1621 ttccacacct cctacaagac caagaagccc gtgaccatgc cccccaacca cgtggtggag
     1681 caccgcatcg ccagaaccga cctggacaag ggcggcaaca gcgtgcagct gaccgagcac
     1741 gccgtggccc acatcacctc cgtggtgccc ttcactagaa tggccctgtc caacaagttc
     1801 atcggcgacg acatgaagat gacctaccac atggacggct gcgtgaacgg ccactacttc
     1861 accgtgaagg gcgagggcag cggcaagccc tacgagggca cccagacctc caccttcaag
     1921 gtgaccatgg ccaacggcgg ccccctggcc ttctccttcg acatcctgtc caccgtgttc
     1981 atgtacggca accgctgctt caccgcctac cccaccagca tgcccgacta cttcaagcag
     2041 gccttccccg acggcatgtc ctacgagaga accttcacct acgaggacgg cggcgtggcc
     2101 accgccagct gggagatcag cctgaagggc aactgcttcg agcacaagtc caccttccac
     2161 ggcgtgaact tccccgccga cggccccgtg atggccaaga agaccaccgg ctgggacccc
     2221 tccttcgaga agatgaccgt gtgcgacggc atcttgaagg gcgacgtgac cgccttcctg
     2281 atgctgcaag gcggcggcaa ctacagatgc cagttccaca cctcctacaa gaccaagaag
     2341 cccgtgacca tgccccccaa ccacgtggtg gagcaccgca tcgccagaac cgacctggac
     2401 aagggcggca acagcgtgca gctgaccgag cacgccgtgg cccacatcac ctccgtggtg
     2461 cccttcacta gacccaagaa aaagcgcaag gtaactagag cgtaaagctg agactagact
     2521 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     2581 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     2641 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     2701 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgg
//