LOCUS       BBa_J176091             2401 bp    DNA     linear       29-May-2024
DEFINITION  HPK-10xGal4-CFP
ACCESSION   BBa_J176091
VERSION     BBa_J176091.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     misc_feature    523..539
                     /label=Gal4
     misc_feature    542..558
                     /label=Gal4
     misc_feature    561..577
                     /label=Gal4
     misc_feature    580..596
                     /label=Gal4
     misc_feature    599..615
                     /label=Gal4
     misc_feature    523..615
                     /label=BBa_J176019
     misc_feature    616..621
                     /label=BBa_J176041
     misc_feature    622..638
                     /label=Gal4
     misc_feature    641..657
                     /label=Gal4
     misc_feature    660..676
                     /label=Gal4
     misc_feature    679..695
                     /label=Gal4
     misc_feature    698..714
                     /label=Gal4
     misc_feature    622..714
                     /label=BBa_J176019
     misc_feature    1..714
                     /label=BBa_S04655
     misc_feature    715..720
                     /label=BBa_J176041
     RBS             721..738
                     /label=BBa_J176012
     misc_feature    739..744
                     /label=BBa_J176041
     misc_feature    745..1431
                     /label=BBa_J176022
     misc_feature    1432..1437
                     /label=BBa_J176041
     misc_feature    1438..2124
                     /label=BBa_J176022
     misc_feature    2125..2130
                     /label=BBa_J176041
     misc_feature    2131..2151
                     /label=BBa_J176008
     misc_feature    2152..2157
                     /label=BBa_J176041
     terminator      2158..2170
                     /label=BBa_J176016
     misc_feature    745..2170
                     /label=BBa_S04684
     misc_feature    721..2170
                     /label=BBa_S04738
     misc_feature    2171..2176
                     /label=BBa_J176041
     misc_feature    2177..2401
                     /label=BBa_J176009
     misc_feature    721..2401
                     /label=BBa_S04739
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga
      541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      601 gagtactgtc ctccgactag acggagtact gtcctccgag cggagtactg tcctccgagc
      661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgactaga
      721 cccgccgcca ccatggagac tagaatggcc ctgtccaaca agttcatcgg cgacgacatg
      781 aagatgacct accacatgga cggctgcgtg aacggccact acttcaccgt gaagggcgag
      841 ggcagcggca agccctacga gggcacccag acctccacct tcaaggtgac catggccaac
      901 ggcggccccc tggccttctc cttcgacatc ctgtccaccg tgttcatgta cggcaaccgc
      961 tgcttcaccg cctaccccac cagcatgccc gactacttca agcaggcctt ccccgacggc
     1021 atgtcctacg agagaacctt cacctacgag gacggcggcg tggccaccgc cagctgggag
     1081 atcagcctga agggcaactg cttcgagcac aagtccacct tccacggcgt gaacttcccc
     1141 gccgacggcc ccgtgatggc caagaagacc accggctggg acccctcctt cgagaagatg
     1201 accgtgtgcg acggcatctt gaagggcgac gtgaccgcct tcctgatgct gcaaggcggc
     1261 ggcaactaca gatgccagtt ccacacctcc tacaagacca agaagcccgt gaccatgccc
     1321 cccaaccacg tggtggagca ccgcatcgcc agaaccgacc tggacaaggg cggcaacagc
     1381 gtgcagctga ccgagcacgc cgtggcccac atcacctccg tggtgccctt cactagaatg
     1441 gccctgtcca acaagttcat cggcgacgac atgaagatga cctaccacat ggacggctgc
     1501 gtgaacggcc actacttcac cgtgaagggc gagggcagcg gcaagcccta cgagggcacc
     1561 cagacctcca ccttcaaggt gaccatggcc aacggcggcc ccctggcctt ctccttcgac
     1621 atcctgtcca ccgtgttcat gtacggcaac cgctgcttca ccgcctaccc caccagcatg
     1681 cccgactact tcaagcaggc cttccccgac ggcatgtcct acgagagaac cttcacctac
     1741 gaggacggcg gcgtggccac cgccagctgg gagatcagcc tgaagggcaa ctgcttcgag
     1801 cacaagtcca ccttccacgg cgtgaacttc cccgccgacg gccccgtgat ggccaagaag
     1861 accaccggct gggacccctc cttcgagaag atgaccgtgt gcgacggcat cttgaagggc
     1921 gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact acagatgcca gttccacacc
     1981 tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc acgtggtgga gcaccgcatc
     2041 gccagaaccg acctggacaa gggcggcaac agcgtgcagc tgaccgagca cgccgtggcc
     2101 cacatcacct ccgtggtgcc cttcactaga cccaagaaaa agcgcaaggt aactagagcg
     2161 taaagctgag actagactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2221 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2281 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2341 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2401 g
//