FLAG

BBa_J18914 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J18914
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-26 12:00:00
Date modified: 2015-08-31 04:08:36

1xFlag tag



    • Details

    Types
    DnaRegion

    Roles
    Tag

    tag

    Sequences BBa_J18914_sequence (Version 1)

    Description

    A FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.

    See also: [[BBa_J64005]]

    Hopp et al. (1988) A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. http://www.nature.com/nbt/journal/v6/n10/abs/nbt1088-1204.html

    Notes

    • gene synthesis


    • codon optimized for E. coli


    Source

    gene synthesis

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J18914/1
     

    Attachments

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