StrepII

BBa_J18916 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18916

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

Strep tag II

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_J18916_sequence (Version 1)

Description

Protein purification tag. Bound by Strepavidine (optimized: strepTactin(tm)) and eluted with biotin. Allows for mild and efficient elution.

SEE ALSO:

• novagen.com, gehealthcare.com (former amersham) for purification protocols

• BBa_I757014, StrepTag II from Freiburg iGem team, has three codon variations

Notes

• gene synthesis

• codon optimized for E. coli

Source

pET52b

gene synthesis

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18916/1

Member of these Collections

• //proteindomain/affinity
• iGEM Parts Registry