LOCUS       BBa_J202042             1830 bp    DNA     linear       18-May-2024
DEFINITION  Ptet Characterization (Mutant#4)
ACCESSION   BBa_J202042
VERSION     BBa_J202042.1  
FEATURES             Location/Qualifiers
     misc_feature    6..6
                     /label=Mutation
     misc_feature    14..14
                     /label=Mutation
     misc_feature    31..31
                     /label=Mutation
     misc_feature    39..39
                     /label=Mutation
     misc_feature    1..19
                     /label=O2
     misc_feature    26..44
                     /label=O2
     promoter        1..54
                     /label=BBa_J202034
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..801
                     /label=GFP protein
     CDS             82..801
                     /label=BBa_E0040
     stem_loop       821..864
                     /label=stem_loop
     misc_feature    810..889
                     /label=BBa_B0010
     terminator      810..889
                     /label=BBa_B0010
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
     misc_feature    63..938
                     /label=BBa_E0240
     promoter        947..981
                     /label=BBa_J23101
     RBS             990..1002
                     /label=RBS-3\Weak
     RBS             996..999
                     /label=RBS
     misc_feature    990..1002
                     /label=BBa_B0032
     RBS             990..1002
                     /label=BBa_B0032
     CDS             1012..1628
                     /label=tetR
     misc_feature    1629..1662
                     /label=SsrA
     misc_feature    1669..1693
                     /label=Help:Barcodes
     CDS             1009..1693
                     /label=BBa_C0040
     stem_loop       1713..1756
                     /label=stem_loop
     misc_feature    1702..1781
                     /label=BBa_B0010
     terminator      1702..1781
                     /label=BBa_B0010
     stem_loop       1797..1816
                     /label=T7 TE
     misc_feature    1823..1823
                     /label=stop
     polyA_site      1817..1830
                     /label=polya
     misc_feature    1790..1830
                     /label=BBa_B0012
     terminator      1790..1830
                     /label=BBa_B0012
     terminator      1702..1830
                     /label=BBa_B0015
ORIGIN
        1 tccctttcag tgaaagagat tgacatccct ttcagtgaaa gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttta cagctagctc
      961 agtcctaggt attatgctag ctactagagt cacacaggaa agtactagat gtccagatta
     1021 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
     1081 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
     1141 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
     1201 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
     1261 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
     1321 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
     1381 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
     1441 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
     1501 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
     1561 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
     1621 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
     1681 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1741 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1801 caccttcggg tgggcctttc tgcgtttata
//