LOCUS BBa_J21005 2829 bp DNA linear 09-May-2024 DEFINITION LxR,LcY,TC ACCESSION BBa_J21005 VERSION BBa_J21005.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..792 /label=mCherry misc_feature 433..433 /label=C->T (removing PstI site) misc_feature 82..795 /label=BBa_J06504 stem_loop 815..858 /label=stem_loop misc_feature 804..883 /label=BBa_B0010 terminator 804..883 /label=BBa_B0010 stem_loop 899..918 /label=T7 TE misc_feature 925..925 /label=stop polyA_site 919..932 /label=polya misc_feature 892..932 /label=BBa_B0012 terminator 892..932 /label=BBa_B0012 misc_feature 943..959 /label=lac O1 promoter 960..965 /label=-35 misc_feature 966..982 /label=lac O1 promoter 983..988 /label=-10 misc_feature 941..994 /label=BBa_R0011 promoter 941..994 /label=BBa_R0011 misc_feature 1008..1011 /label=conserved RBS 1004..1015 /label=BBa_B0034 CDS 1022..1744 /label=BBa_E0030 stem_loop 1764..1807 /label=stem_loop misc_feature 1753..1832 /label=BBa_B0010 terminator 1753..1832 /label=BBa_B0010 stem_loop 1848..1867 /label=T7 TE misc_feature 1874..1874 /label=stop polyA_site 1868..1881 /label=polya misc_feature 1841..1881 /label=BBa_B0012 terminator 1841..1881 /label=BBa_B0012 misc_feature 1890..1908 /label=TetR 1 misc_feature 1890..1943 /label=BBa_R0040 promoter 1909..1914 /label=-35 misc_feature 1915..1933 /label=TetR 2 promoter 1932..1937 /label=-10 promoter 1890..1943 /label=BBa_R0040 misc_feature 1956..1959 /label=conserved RBS 1952..1963 /label=BBa_B0034 CDS 1970..2692 /label=BBa_E0020 stem_loop 2712..2755 /label=stem_loop misc_feature 2701..2780 /label=BBa_B0010 terminator 2701..2780 /label=BBa_B0010 stem_loop 2796..2815 /label=T7 TE misc_feature 2822..2822 /label=stop polyA_site 2816..2829 /label=polya misc_feature 2789..2829 /label=BBa_B0012 terminator 2789..2829 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc 121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 781 ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat 961 tgacattgtg agcggataac aagatactga gcacatacta gagaaagagg agaaatacta 1021 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 1081 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 1141 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 1201 cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg accacatgaa 1261 gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 1321 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 1381 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 1441 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 1501 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 1561 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 1621 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 1681 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 1741 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 1801 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 1861 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta 1921 tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga tggtgagcaa 1981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa 2041 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 2101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 2161 cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 2221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 2281 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 2341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 2401 caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc 2461 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 2521 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 2581 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 2641 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat aatactagag 2701 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 2761 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 2821 gcgtttata //