LOCUS       BBa_J22081              1044 bp    DNA     linear       30-Apr-2024
DEFINITION  pLac pLux YFP(+LVA)
ACCESSION   BBa_J22081
VERSION     BBa_J22081.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    64..83
                     /label=LuxR/HSL
     promoter        83..88
                     /label=-35
     promoter        105..110
                     /label=-10
     misc_feature    116..116
                     /label=start
     misc_feature    64..118
                     /label=BBa_R0062
     promoter        64..118
                     /label=BBa_R0062
     RBS             127..139
                     /label=RBS-3\Weak
     RBS             133..136
                     /label=RBS
     misc_feature    127..139
                     /label=BBa_B0032
     RBS             127..139
                     /label=BBa_B0032
     CDS             146..907
                     /label=YFP (LVA)
     misc_feature    902..907
                     /label=2
     misc_feature    863..901
                     /label=SsrA
     misc_feature    387..387
                     /label=A (Q->L)
     misc_feature    146..907
                     /label=BBa_E0032
     misc_feature    146..907
                     /label=BBa_E0032
     stem_loop       927..970
                     /label=stem_loop
     misc_feature    916..995
                     /label=BBa_B0010
     terminator      916..995
                     /label=BBa_B0010
     stem_loop       1011..1030
                     /label=T7 TE
     misc_feature    1037..1037
                     /label=stop
     polyA_site      1031..1044
                     /label=polya
     misc_feature    1004..1044
                     /label=BBa_B0012
     terminator      1004..1044
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaata
      121 ctagagtcac acaggaaagt actagatggt gagcaagggc gaggagctgt tcaccggggt
      181 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      241 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      301 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt
      361 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg
      421 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      481 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      541 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      601 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      661 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      721 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
      781 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      841 cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc
      901 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      961 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1021 cgggtgggcc tttctgcgtt tata
//