LOCUS       BBa_J22281              2697 bp    DNA     linear       28-May-2024
DEFINITION  J22261 J22221
ACCESSION   BBa_J22281
VERSION     BBa_J22281.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     misc_feature    64..862
                     /label=BBa_S01640
     misc_feature    875..878
                     /label=conserved
     RBS             871..882
                     /label=BBa_B0034
     CDS             889..1611
                     /label=BBa_E0030
     stem_loop       1631..1674
                     /label=stem_loop
     misc_feature    1620..1699
                     /label=BBa_B0010
     terminator      1620..1699
                     /label=BBa_B0010
     stem_loop       1715..1734
                     /label=T7 TE
     misc_feature    1741..1741
                     /label=stop
     polyA_site      1735..1748
                     /label=polya
     misc_feature    1708..1748
                     /label=BBa_B0012
     terminator      1708..1748
                     /label=BBa_B0012
     misc_feature    871..1748
                     /label=BBa_E0430
     misc_feature    64..1748
                     /label=BBa_J22211
     misc_feature    1..1748
                     /label=BBa_J22261
     misc_feature    1757..1776
                     /label=LuxR/HSL
     promoter        1776..1781
                     /label=-35
     promoter        1798..1803
                     /label=-10
     misc_feature    1809..1809
                     /label=start
     misc_feature    1757..1811
                     /label=BBa_R0062
     promoter        1757..1811
                     /label=BBa_R0062
     misc_feature    1824..1827
                     /label=conserved
     RBS             1820..1831
                     /label=BBa_B0034
     CDS             1838..2560
                     /label=BBa_E0020
     stem_loop       2580..2623
                     /label=stem_loop
     misc_feature    2569..2648
                     /label=BBa_B0010
     terminator      2569..2648
                     /label=BBa_B0010
     stem_loop       2664..2683
                     /label=T7 TE
     misc_feature    2690..2690
                     /label=stop
     polyA_site      2684..2697
                     /label=polya
     misc_feature    2657..2697
                     /label=BBa_B0012
     terminator      2657..2697
                     /label=BBa_B0012
     misc_feature    1757..2697
                     /label=BBa_J22221
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat ggtgagcaag
      901 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      961 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1021 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1081 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc
     1141 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1201 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1261 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1321 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1381 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1441 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     1501 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1561 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc
     1621 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1681 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1741 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat
     1801 agtcgaataa atactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct
     1861 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
     1921 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1981 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
     2041 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2101 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2161 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2221 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
     2281 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     2341 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2401 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2461 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2521 cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa
     2581 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2641 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//