BBa_J23005 1 BBa_J23005 [FRT-CmR-FRT] 2006-06-22T11:00:00Z 2015-08-31T04:08:38Z <tt> PCR ca1005F/R on pKD3 (1066 bp, EcoRI/PstI)<br> sub into pSB1A2 (EcoRI/PstI)<br> product is pSB1A2-Bca1005<br> ---- ca1005F Forward Biobrick for pKD3 FRT-Cm-FRT<br> gacttgaattcgcggccgcttctagagtgtaggctggagctgcttc<br> ca1005R Reverse Biobrick for pKD3 FRT-Cm-FRT<br> cctaactgcagcggccgctactagtacatatgaatatcctccttag<br> </tt> Biobricked cassette containing the chloramphenicol resistance gene flanked by FRT sites. Useful for constructing markerless genome-integration cassettes. The cassette is derived from pKD3 described by Datsenko&Wanner and can be excised by expression of flp recombinase in trans with helper plasmids such as pCP20. true false _52_ 0 483 52 Discontinued false N/A false John Anderson BBa_J23005_sequence 1 tgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcggaataggaacttcatttaaatggcgcgccttacgccccgccctgccactcatcgcagtactgttgtattcattaagcatctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgtaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgacaactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtaggcgcgccgaagttcctatactttctagagaataggaacttcggaataggaactaaggaggatattcatatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z