BBa_J23008

BBa_J23008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J23008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kaitlin Davis
Date created: 2006-07-09 11:00:00
Date modified: 2015-08-31 04:08:38

[key3c] riboregulator for lock3 variants



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_J23008_sequence (Version 1)

Description

This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.

Notes

No design considerations

Source

J23007

igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J23008/1