LOCUS       BBa_J23029               962 bp    DNA     linear       17-May-2024
DEFINITION  [Ptet][key3][StrRBS][luxI][TT]
ACCESSION   BBa_J23029
VERSION     BBa_J23029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..156
                     /label=BBa_J01086
     misc_feature    1..156
                     /label=BBa_J01129
     misc_feature    169..172
                     /label=conserved
     RBS             165..176
                     /label=BBa_B0034
     misc_feature    762..793
                     /label=LVA
     CDS             183..761
                     /label=luxI
     misc_feature    183..800
                     /label=BBa_C0061
     misc_feature    801..825
                     /label=Help:Barcodes
     CDS             183..800
                     /label=BBa_C0061
     stem_loop       845..888
                     /label=stem_loop
     misc_feature    834..913
                     /label=BBa_B0010
     terminator      834..913
                     /label=BBa_B0010
     stem_loop       929..948
                     /label=T7 TE
     misc_feature    955..955
                     /label=stop
     polyA_site      949..962
                     /label=polya
     misc_feature    922..962
                     /label=BBa_B0012
     terminator      922..962
                     /label=BBa_B0012
     misc_feature    165..962
                     /label=BBa_F1610
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agacccaaaa gcaggaggtg aatctagtag gtggttaatg aaaattaact tacttactag
      121 aaatatctct aaaaagccag attattaatc cggctttact agagaaagag gagaaatact
      181 agatgactat aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag
      241 gtattctaag tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag
      301 aaaataacct tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg
      361 atgatactga aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc
      421 tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag
      481 tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg
      541 aaattacaat gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag
      601 aatatgtaac agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt
      661 gtcatcgtat tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt
      721 ctatgcctat taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact
      781 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca
      841 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      901 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      961 ta
//