LOCUS       BBa_J23049              2349 bp    DNA     linear       04-May-2024
DEFINITION  [lock3d][RFP]
ACCESSION   BBa_J23049
VERSION     BBa_J23049.1  
FEATURES             Location/Qualifiers
     misc_feature    1..43
                     /label=BBa_J23032
     misc_feature    2131..2259
                     /label=BBa_B0015 terminator
     misc_feature    2268..2321
                     /label=BBa_R0040 (Ptet)
     misc_feature    234..853
                     /label=pBR322 origin
     misc_feature    231..946
                     /label=pMB1 origin
     CDS             1008..1868
                     /label=beta-lactamase (ampicillin resistance marker)
     promoter        1910..1938
                     /label=beta-lactamase (ampR) promoter
     stem_loop       2142..2185
                     /label=rrnB T1 terminator
     misc_feature    52..72
                     /label=BioBrick suffix
     misc_feature    2109..2114
                     /label=EcoRI
     misc_feature    2115..2122
                     /label=NotI
     misc_feature    2343..2348
                     /label=XbaI
     primer_bind     1991..2010
                     /label=Verification forward (VF2) primer binding site
     primer_bind     130..149
                     /label=Verification reverse (VR) primer binding site
     misc_feature    2330..2341
                     /label=BBa_B0034
     RBS             2330..2341
                     /label=RBS
     promoter        2268..2321
                     /label=Ptet
     misc_feature    52..2349
                     /label=BBa_J23018
ORIGIN
        1 aactagaatc acctcttgct tttgggtaag acagaagagg agatactaga gtactagtag
       61 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      121 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      181 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      661 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
      721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1981 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2041 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2101 gatttctgga attcgcggcc gcaactagag ccaggcatca aataaaacga aaggctcagt
     2161 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2221 acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga
     2281 tagagattga catccctatc agtgatagag atactgagca ctactagaga aagaggagaa
     2341 attctagag
//