LOCUS       BBa_J23050              2346 bp    DNA     linear       02-May-2024
DEFINITION  [lock3e][RFP]
ACCESSION   BBa_J23050
VERSION     BBa_J23050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_J23033
     misc_feature    2128..2256
                     /label=BBa_B0015 terminator
     misc_feature    2265..2318
                     /label=BBa_R0040 (Ptet)
     misc_feature    231..850
                     /label=pBR322 origin
     misc_feature    228..943
                     /label=pMB1 origin
     CDS             1005..1865
                     /label=beta-lactamase (ampicillin resistance marker)
     promoter        1907..1935
                     /label=beta-lactamase (ampR) promoter
     stem_loop       2139..2182
                     /label=rrnB T1 terminator
     misc_feature    49..69
                     /label=BioBrick suffix
     misc_feature    2106..2111
                     /label=EcoRI
     misc_feature    2112..2119
                     /label=NotI
     misc_feature    2340..2345
                     /label=XbaI
     primer_bind     1988..2007
                     /label=Verification forward (VF2) primer binding site
     primer_bind     127..146
                     /label=Verification reverse (VR) primer binding site
     misc_feature    2327..2338
                     /label=BBa_B0034
     RBS             2327..2338
                     /label=RBS
     promoter        2265..2318
                     /label=Ptet
     misc_feature    49..2346
                     /label=BBa_J23018
ORIGIN
        1 aactagaatc aactcttgct tttgggtaag aaagaggaga tactagagta ctagtagcgg
       61 ccgctgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      661 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
      721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1141 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1981 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2041 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
     2101 ttctggaatt cgcggccgca actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2161 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2221 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag
     2281 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaatt
     2341 ctagag
//