BBa_J23068

BBa_J23068 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J23068
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-08-13 11:00:00
Date modified: 2015-08-31 04:08:39

Intermediate #2 in construction of TraGr cassette



Types
DnaRegion

Roles
Intermediate

engineered_region

Sequences BBa_J23068_sequence (Version 1)

Description

TraG region (J23068) cloned into pSB1A2-Bca9007

Notes

n/a

Source


PCR JL20/JL21 on RP4 gen. (2268 bp, MfeI/BglII)

Sub into pGLN (BamHI/EcoRI)

Product is pJL20(J23067)



IPCR JL22/JL23 on pJL20(J23067) (3454 bp, EcoRI/PstI)

Sub in pSB1A2-Bca9007 (1015 bp, EcoRI/PstI)

Product is pJL22(J23068)



PCR JL18/JL19 on pJL22(J23068) for D/W (bp, DpnI)

Datsenko Wanner on pKD46/Rlambda,

Product is J23069



JL20 Forward BglII to BamHI for traG-5' knockout cassette

GAGTCAGATCTgattatgttggtgcgctg

JL21 Reverse MfeI to EcoRI for traG-5' knockout cassette

gctggCAATTGcattcgatttccagtacaggc

JL22 Reverse EcoRI IPCR of traG

gctgcGAATTCgatactggaaggaatgggc

JL23 Forward PstI IPCR of traG

cattgCTGCAGacggcagcattgaagaggcg

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J23068/1