LOCUS       BBa_J23074              2113 bp    DNA     linear       15-May-2024
DEFINITION  [RA2 Promoter][RBS][TraGr][TT]
ACCESSION   BBa_J23074
VERSION     BBa_J23074.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1976
                     /label=BBa_J23013
     stem_loop       1996..2039
                     /label=stem_loop
     misc_feature    1985..2064
                     /label=BBa_B0010
     terminator      1985..2064
                     /label=BBa_B0010
     stem_loop       2080..2099
                     /label=T7 TE
     misc_feature    2106..2106
                     /label=stop
     polyA_site      2100..2113
                     /label=polya
     misc_feature    2073..2113
                     /label=BBa_B0012
     terminator      2073..2113
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgaagaac cgaaacaacg ccgtggggcc acagatacgg gcgaaaaaac cgaaggccag
      121 caagaccgtt cccatcctcg ccggcctgtc ccttggggca ggcttgcaga ccgcgacgca
      181 atatttcgcc cattccttcc agtatcaggc cggacttggc tggaatatca accacgtcta
      241 cacgccctgg tcgatccttc aatgggccgg caagtggtac ggccaatatc ccgacgattt
      301 catgcgcgcg gccagcatgg gtatggtcgt ttcgaccgtg ggcctgctgg gcacggccgt
      361 cacgcagatg gtcaaggcga acaccggcaa ggcgaacgac tatctgcacg gttcggcccg
      421 ctgggccgac aagaaggaca tacaggccgc cggcctgctg ccccggccgc gcaccgtcgt
      481 cgagctggtg tcaggcaaac acccgcccac ttccagcggc gtctatgtgg gcggctggca
      541 ggacaaggac ggcaagttcc actacctgcg gcacaacggc cccgagcacg tcttgaccta
      601 cgcgccgacg cgctccggca agggcgtcgg cctggtcgtt ccgacgctgc tttcctgggc
      661 gcacagcgcc gtcatcaccg acctgaaagg cgagttgtgg gcgctgaccg ccggctggcg
      721 gaagaagcat gcccgcaaca aggtcgtgcg ctttgagccg gcatccgcgc agggtagcgc
      781 gtgctggaac ccgctcgatg aaatccgctt ggggaccgag tacgaggttg gcgacgtgca
      841 gaaccttgcc accctgatcg tcgatccgga cggcaagggc ctggaatcgc actggcagaa
      901 aaccagccag gcgctgcttg tcggcgtcat cctgcacgcg ctctacaagg ctaagaacga
      961 gggtacgccg gccaccctgc cgtcggtgga cggcatgctt gccgacccga accgcgacgt
     1021 gggcgagctt tggatggaaa tgaccaccta cggccacgtt gacgggcaga accaccctgc
     1081 ggtcggctct gcggcccgcg acatgatgga ccgcccggag gaagaatccg gttccgtgct
     1141 gtccaccgcc aagtcctacc tggccctgta ccgcgacccg gttgtggccc gcaacgtcag
     1201 caagtccgac ttccgcatca agcaactgat gcaccacgac gacccggtaa gcctgttcat
     1261 cgtgacgcag cccaacgaca aggcccgtct gcggcctctg gtgcgcgtca tggtcaacat
     1321 gatcgtccgg ctgctggccg acaagatgga cttcgagaac ggccgtcctg tcgcgcacta
     1381 caagcatcgt ctgctgatga tgctcgacga gttccccagc ctgggcaagc tcgaaatcct
     1441 gcaagagtcc cttgccttcg tcgccggcta cggcatcaag tgctacctca tctgccagga
     1501 cattaaccag ctcaagagcc gcgaaaccgg ctacggccac gacgaaagca tcacgtcgaa
     1561 ctgccacgtg cagaacgcct acccgccgaa ccgcgtggag acggccgagc acctgtcaaa
     1621 actgacaggc accacgacca tcgtgaagga gcagatcacg acgagcggcc gccgcacgtc
     1681 ggcgctgttg ggcaacgtct cgcggacctt ccaggaagtg cagcggccat tgctgacccc
     1741 cgatgaatgc ctgcgcatgc cggggccgaa gaagagcgcc gacggcagca ttgaagaggc
     1801 gggcgacatg gttgtgtacg tcgccggcta tcccgcgatc tacggcaagc agccgctcta
     1861 cttcaaagac ccgatattcc aggcccgcgc ggccgtaccg gctccgaagg tcagcgacaa
     1921 gctgatccag acggccaccg tcgaggaagg ggaggggatc acgatatgag ccgctttact
     1981 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2041 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2101 ttctgcgttt ata
//