BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J23084 1 BBa_J23084 [Ser2 tRNA key(-18)][b0015] 2006-11-14T12:00:00Z 2015-08-31T04:08:40Z j23096 and b0015 j23096 with b0015 added to the 5' end. false false _52_ 0 936 52 Not in stock false the presence of terminators on the 5' end of ribopregulator keys have consistently shown to produce a 2 fold improvement in activation potential. false bryan hernandez component1910479 1 BBa_B0012 component1910476 1 BBa_J23096 component1910477 1 BBa_B0010 annotation1910476 1 BBa_J23096 range1910476 1 1 130 annotation1910479 1 BBa_B0012 range1910479 1 227 267 annotation1910477 1 BBa_B0010 range1910477 1 139 218 BBa_J23096 1 BBa_J23096 Ser2 tRNA key(-18) 2006-11-14T12:00:00Z 2015-08-31T04:08:40Z ser2 tRNA this is a ser2 derived tRNA key with an addressive sequence that anneals 15 bases past the 5' most part of j23077 lock's rbs' while still completely covering the rbs'. the 3' most base on the lock's rbs' will be called base (0). so key(0)'s 3'-most binding occurs at base (0) on the lock. key(n)'s 3' most binding occurs at base n. rbs' is the complement of the RBS in the lock's hairpin. false false _52_ 0 936 52 Not in stock false this is a variant of keys designed to test the key's activation potential with respect to its specific annealling location on the lock while still completely duplexing with the rbs' false bryan hernandez BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J23084_sequence 1 ggagagatgccggagcggctgaacggaccgggatccataaaagtcttacccaaaagcaagaggtgattcaaaaaagcttcccggagtaggggcaactctaccgggggttcaaatccccctctctccgccatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23096_sequence 1 ggagagatgccggagcggctgaacggaccgggatccataaaagtcttacccaaaagcaagaggtgattcaaaaaagcttcccggagtaggggcaactctaccgggggttcaaatccccctctctccgcca BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z