LOCUS       BBa_J24014              1837 bp    DNA     linear       14-May-2024
DEFINITION  Toggle Switch to Immobilize E. Coli
ACCESSION   BBa_J24014
VERSION     BBa_J24014.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..219
                     /label=RBS-4\Weaker
     RBS             215..218
                     /label=RBS
     misc_feature    209..219
                     /label=BBa_B0033
     RBS             209..219
                     /label=BBa_B0033
     CDS             226..828
                     /label=LasI
     misc_feature    829..861
                     /label=LVA
     misc_feature    868..892
                     /label=Help:Barcodes
     CDS             226..892
                     /label=BBa_C0078
     CDS             901..1837
                     /label=BBa_S03271
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggact actagatgat cgttcagatc
      241 ggtcgtcgtg aagagttcga caaaaaactg ctgggtgaaa tgcacaaact gcgtgctcag
      301 gttttcaaag aacgtaaagg ttgggacgtt tccgttatcg acgaaatgga aatcgacggt
      361 tacgacgctc tgtccccgta ctacatgctg atccaggaag acaccccgga agctcaggtt
      421 ttcggttgct ggcgtatctt cgacaccacc ggtccgtaca tgctgaaaaa caccttcccg
      481 gaactgctgc acggtaaaga agctccgtgc tccccgcaca tctgggaact gtcccgtttc
      541 gctatcaact ccggtcagaa aggttccctg ggtttctccg actgcaccct ggaagctatg
      601 cgtgctctgg ctcgttactc cttgcagaac gacatccaga ccctggttac cgttaccacc
      661 gttggtgttg aaaaaatgat gatccgtgct ggtctggacg tttcccgttt cggtccgcac
      721 ctgaaaatcg gtatcgaacg tgctgttgct ctgcgtatcg aactgaacgc taaaacccag
      781 atcgctctgt acggtggtgt tctggttgaa cagcgtctgg ctgtttccgc tgctaacgac
      841 gaaaactacg ctctggttgc ttaataactc tgatagtgct agtgtagatc tctactagag
      901 ccgaggaagc atgaagaatc aagcgcatcc gattattgtc gtcaaacgac gcaaagccaa
      961 aagccacggg gcagcacatg gatcgtggaa gattgcttat gccgacttta tgactgcgat
     1021 gatggccttt tttctggtga tgtggctgat ctccatctcc agcccaaaag agctgattca
     1081 gattgcggag tacttccgga ctccactggc gactgcggtt acgggcggcg atcgcatttc
     1141 taatagtgaa agcccaattc ccggcggtgg tgatgattac acccaaagcc agggggaagt
     1201 gaataagcag ccgaacatcg aagagctgaa aaaacgcatg gagcaaagtc gattgcggaa
     1261 attgcggggt gatctcgacc agttgataga gtccgatccg aaactgcggg cgttacgtcc
     1321 ccatctcaaa atcgatctgg tccaggaagg tctacgtatt cagatcatcg atagccagaa
     1381 tcgcccgatg tttagaaccg gcagtgccga tgtcgaaccc tatatgcgcg acattctgcg
     1441 cgccattgcg cctgtactga acggtattcc caaccgtatt agcctttcag gtcataccga
     1501 tgatttcccc tacgccagcg gtgagaaagg atatagcaac tgggagcttt ctgccgatcg
     1561 ggccaatgca tcccgccgcg aactgatggt cggagggttg gatagcggca aagtgttacg
     1621 tgtcgtcggc atggcggcaa cgatgcgctt aagcgatcgc ggacctgatg atgccgtcaa
     1681 ccgtcgcatc agcctgctgg tactgaacaa acaagccgaa caggccattt tgcatgaaaa
     1741 cgccgaaagc cagaatgagc cagtaagcgc cctggaaaaa cctgaggttg caccacaggt
     1801 cagtgttccc acaatgccat cagccgaacc gaggtga
//