LOCUS       BBa_J24667              1562 bp    DNA     linear       13-May-2024
DEFINITION  TetR repressed LuxI / RFP Generator
ACCESSION   BBa_J24667
VERSION     BBa_J24667.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    87..90
                     /label=conserved
     RBS             83..94
                     /label=BBa_B0034
     CDS             101..685
                     /label=LuxI
     CDS             101..685
                     /label=BBa_C0161
     misc_feature    698..701
                     /label=conserved
     RBS             694..705
                     /label=BBa_B0034
     CDS             712..1422
                     /label=mCherry
     misc_feature    1063..1063
                     /label=C->T (removing PstI site)
     misc_feature    712..1425
                     /label=BBa_J06504
     stem_loop       1445..1488
                     /label=stem_loop
     misc_feature    1434..1513
                     /label=BBa_B0010
     terminator      1434..1513
                     /label=BBa_B0010
     stem_loop       1529..1548
                     /label=T7 TE
     misc_feature    1555..1555
                     /label=stop
     polyA_site      1549..1562
                     /label=polya
     misc_feature    1522..1562
                     /label=BBa_B0012
     terminator      1522..1562
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agaaagagga gaaatactag atgactataa tgataaaaaa
      121 atcggatttt ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca
      181 agtgtttaag caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga
      241 gtatgataac tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg
      301 atgctggcgt ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt
      361 gcttggtcaa cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt
      421 aggtaaaaat agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga
      481 agctatatat aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac
      541 agcaatagag cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga
      601 aattcatgta ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt
      661 taaaaaagca gtcttaaatt aataatacta gagaaagagg agaaatacta gatggtgagc
      721 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      781 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      841 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      901 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      961 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     1021 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccttgcagga cggcgagttc
     1081 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     1141 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     1201 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     1261 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     1321 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     1381 ggccgccact ccaccggcgg catggacgag ctgtacaagt aataatacta gagccaggca
     1441 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1501 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1561 ta
//