LOCUS       BBa_J24668              1542 bp    DNA     linear       19-May-2024
DEFINITION  TetR regulated AHL Producer (with LVA- RFP)
ACCESSION   BBa_J24668
VERSION     BBa_J24668.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..665
                     /label=LuxI
     CDS             81..665
                     /label=BBa_C0161
     misc_feature    678..681
                     /label=conserved
     RBS             674..685
                     /label=BBa_B0034
     CDS             692..1402
                     /label=mCherry
     misc_feature    1043..1043
                     /label=C->T (removing PstI site)
     misc_feature    692..1405
                     /label=BBa_J06504
     stem_loop       1425..1468
                     /label=stem_loop
     misc_feature    1414..1493
                     /label=BBa_B0010
     terminator      1414..1493
                     /label=BBa_B0010
     stem_loop       1509..1528
                     /label=T7 TE
     misc_feature    1535..1535
                     /label=stop
     polyA_site      1529..1542
                     /label=polya
     misc_feature    1502..1542
                     /label=BBa_B0012
     terminator      1502..1542
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc
      121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg
      181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag
      241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta
      301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc
      361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga
      421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg
      481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa
      541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata
      601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatt
      661 aataatacta gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat
      721 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      781 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      841 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      901 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      961 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1021 gaccgtgacc caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg
     1081 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1141 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1201 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1261 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1321 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1381 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1441 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1501 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//