LOCUS BBa_J24676 3977 bp DNA linear 04-May-2024 DEFINITION pBAD to tetR coding region, lacI coding region, and mCherry reporter ACCESSION BBa_J24676 VERSION BBa_J24676.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1240..1856 /label=tetR misc_feature 1857..1890 /label=SsrA misc_feature 1897..1921 /label=Help:Barcodes CDS 1237..1921 /label=BBa_C0040 misc_feature 1219..1921 /label=BBa_S03518 misc_feature 1934..1937 /label=conserved RBS 1930..1941 /label=BBa_B0034 misc_feature 3037..3075 /label=LVA CDS 1948..3075 /label=lacI-LVA misc_feature 1948..3075 /label=BBa_C0012 misc_feature 3076..3100 /label=Help:Barcodes CDS 1948..3075 /label=BBa_C0012 misc_feature 1930..3100 /label=BBa_S03520 misc_feature 3113..3116 /label=conserved RBS 3109..3120 /label=BBa_B0034 CDS 3127..3837 /label=mCherry misc_feature 3478..3478 /label=C->T (removing PstI site) misc_feature 3127..3840 /label=BBa_J06504 stem_loop 3860..3903 /label=stem_loop misc_feature 3849..3928 /label=BBa_B0010 terminator 3849..3928 /label=BBa_B0010 stem_loop 3944..3963 /label=T7 TE misc_feature 3970..3970 /label=stop polyA_site 3964..3977 /label=polya misc_feature 3937..3977 /label=BBa_B0012 terminator 3937..3977 /label=BBa_B0012 misc_feature 3109..3977 /label=BBa_J06702 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa 1261 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 1321 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 1381 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 1441 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 1501 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 1561 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 1621 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 1681 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 1741 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 1801 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 1861 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 1921 ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac gttatacgat 1981 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 2041 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 2101 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 2161 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 2221 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 2281 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 2341 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 2401 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 2461 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 2521 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 2581 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 2641 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 2701 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 2761 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 2821 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 2881 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 2941 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 3001 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct 3061 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa agaggagaaa 3121 tactagatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 3181 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 3241 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 3301 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 3361 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 3421 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg 3481 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 3541 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 3601 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 3661 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 3721 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 3781 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaataa 3841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3961 gcctttctgc gtttata //