LOCUS       BBa_J24709              1629 bp    DNA     linear       13-May-2024
DEFINITION  29. M RBS - TetR - S RBS - ECFP (+LVA) - terminator
ACCESSION   BBa_J24709
VERSION     BBa_J24709.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             23..639
                     /label=tetR
     misc_feature    640..673
                     /label=SsrA
     misc_feature    680..704
                     /label=Help:Barcodes
     CDS             20..704
                     /label=BBa_C0040
     misc_feature    1..704
                     /label=BBa_J24687
     misc_feature    717..720
                     /label=conserved
     RBS             713..724
                     /label=BBa_B0034
     CDS             731..1492
                     /label=CFP (LVA)
     misc_feature    1449..1486
                     /label=SsrA
     misc_feature    1487..1492
                     /label=2
     misc_feature    799..799
                     /label=A
     misc_feature    731..1492
                     /label=BBa_E0022
     misc_feature    731..1492
                     /label=BBa_E0022
     stem_loop       1512..1555
                     /label=stem_loop
     misc_feature    1501..1580
                     /label=BBa_B0010
     terminator      1501..1580
                     /label=BBa_B0010
     stem_loop       1596..1615
                     /label=T7 TE
     misc_feature    1622..1622
                     /label=stop
     polyA_site      1616..1629
                     /label=polya
     misc_feature    1589..1629
                     /label=BBa_B0012
     terminator      1589..1629
                     /label=BBa_B0012
     misc_feature    713..1629
                     /label=BBa_J24703
ORIGIN
        1 tcacacagga aagtactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
       61 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      121 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
      181 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      241 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      301 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
      361 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
      421 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
      481 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
      541 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
      601 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
      661 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agaaagagga
      721 gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      781 cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      841 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      901 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga
      961 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1021 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1081 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1141 cctggggcac aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa
     1201 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
     1261 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1321 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     1381 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1441 gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag
     1501 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1561 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1621 gcgtttata
//