LOCUS       BBa_J24710              1628 bp    DNA     linear       17-May-2024
DEFINITION  30. S RBS - TetR - S RBS - ECFP (+LVA) - terminator
ACCESSION   BBa_J24710
VERSION     BBa_J24710.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..703
                     /label=BBa_C0040
     misc_feature    1..703
                     /label=BBa_J24688
     misc_feature    716..719
                     /label=conserved
     RBS             712..723
                     /label=BBa_B0034
     CDS             730..1491
                     /label=CFP (LVA)
     misc_feature    1448..1485
                     /label=SsrA
     misc_feature    1486..1491
                     /label=2
     misc_feature    798..798
                     /label=A
     misc_feature    730..1491
                     /label=BBa_E0022
     misc_feature    730..1491
                     /label=BBa_E0022
     stem_loop       1511..1554
                     /label=stem_loop
     misc_feature    1500..1579
                     /label=BBa_B0010
     terminator      1500..1579
                     /label=BBa_B0010
     stem_loop       1595..1614
                     /label=T7 TE
     misc_feature    1621..1621
                     /label=stop
     polyA_site      1615..1628
                     /label=polya
     misc_feature    1588..1628
                     /label=BBa_B0012
     terminator      1588..1628
                     /label=BBa_B0012
     misc_feature    712..1628
                     /label=BBa_J24703
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag
      721 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      781 gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      841 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      901 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac
      961 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1021 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1081 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1141 ctggggcaca agctggagta caactacatc agccacaacg tctatatcac cgccgacaag
     1201 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     1261 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1321 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1381 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1441 tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc
     1501 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1561 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1621 cgtttata
//