LOCUS       BBa_J24718              2765 bp    DNA     linear       25-May-2024
DEFINITION  38. pLacI=M RBS=AraC=M RBS=TetR=Strong RBS=mCherry(+LVA)=terminator
ACCESSION   BBa_J24718
VERSION     BBa_J24718.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..1103
                     /label=araC
     misc_feature    1104..1136
                     /label=LVA
     misc_feature    1143..1167
                     /label=Help:Barcodes
     CDS             228..1167
                     /label=BBa_C0080
     misc_feature    1..1167
                     /label=BBa_J24692
     RBS             1176..1188
                     /label=RBS-3\Weak
     RBS             1182..1185
                     /label=RBS
     misc_feature    1176..1188
                     /label=BBa_B0032
     RBS             1176..1188
                     /label=BBa_B0032
     CDS             1198..1814
                     /label=tetR
     misc_feature    1815..1848
                     /label=SsrA
     misc_feature    1855..1879
                     /label=Help:Barcodes
     CDS             1195..1879
                     /label=BBa_C0040
     misc_feature    1176..1879
                     /label=BBa_J24687
     misc_feature    1892..1895
                     /label=conserved
     RBS             1888..1899
                     /label=BBa_B0034
     CDS             1906..2613
                     /label=mCherry
     misc_feature    2257..2257
                     /label=C->T (removing PstI site)
     misc_feature    2614..2622
                     /label=LVA
     misc_feature    1906..2628
                     /label=BBa_J06505
     stem_loop       2648..2691
                     /label=stem_loop
     misc_feature    2637..2716
                     /label=BBa_B0010
     terminator      2637..2716
                     /label=BBa_B0010
     stem_loop       2732..2751
                     /label=T7 TE
     misc_feature    2758..2758
                     /label=stop
     polyA_site      2752..2765
                     /label=polya
     misc_feature    2725..2765
                     /label=BBa_B0012
     terminator      2725..2765
                     /label=BBa_B0012
     misc_feature    1888..2765
                     /label=BBa_J24702
     misc_feature    1176..2765
                     /label=BBa_J24706
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gctgaagcgc
      241 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc
      301 cgattgaggc caatggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt
      361 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg
      421 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc
      481 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc
      541 atgaatggct taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag
      601 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag
      661 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg
      721 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt
      781 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt
      841 gcctgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa
      901 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcact acccggatgc
      961 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat
     1021 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga
     1081 atgatgtagc cgtcaagttg tcagctgcaa acgacgaaaa ctacgcttta gtagcttaat
     1141 aacactgata gtgctagtgt agatcactac tagagtcaca caggaaagta ctagatgtcc
     1201 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
     1261 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
     1321 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
     1381 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
     1441 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     1501 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     1561 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     1621 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     1681 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     1741 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     1801 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga
     1861 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggt gagcaagggc
     1921 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1981 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2041 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2101 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2161 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2221 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac
     2281 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2341 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2401 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2461 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2521 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2581 cactccaccg gcggcatgga cgagctgtac aagttagtag cttaataata ctagagccag
     2641 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2701 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2761 ttata
//