LOCUS BBa_J24723 3128 bp DNA linear 02-May-2024 DEFINITION 43. 16(BBa_J24694)==31(BBa_J24711) ACCESSION BBa_J24723 VERSION BBa_J24723.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 69..72 /label=conserved RBS 63..76 /label=BBa_B0031 CDS 83..958 /label=araC misc_feature 959..991 /label=LVA misc_feature 998..1022 /label=Help:Barcodes CDS 83..1022 /label=BBa_C0080 misc_feature 1..1022 /label=BBa_J24694 misc_feature 1037..1040 /label=conserved RBS 1031..1044 /label=BBa_B0031 misc_feature 2140..2178 /label=LVA CDS 1051..2178 /label=lacI-LVA misc_feature 1051..2178 /label=BBa_C0012 misc_feature 2179..2203 /label=Help:Barcodes CDS 1051..2178 /label=BBa_C0012 misc_feature 1031..2203 /label=BBa_J24683 misc_feature 2216..2219 /label=conserved RBS 2212..2223 /label=BBa_B0034 CDS 2230..2991 /label=YFP (LVA) misc_feature 2986..2991 /label=2 misc_feature 2947..2985 /label=SsrA misc_feature 2471..2471 /label=A (Q->L) misc_feature 2230..2991 /label=BBa_E0032 misc_feature 2230..2991 /label=BBa_E0032 stem_loop 3011..3054 /label=stem_loop misc_feature 3000..3079 /label=BBa_B0010 terminator 3000..3079 /label=BBa_B0010 stem_loop 3095..3114 /label=T7 TE misc_feature 3121..3121 /label=stop polyA_site 3115..3128 /label=polya misc_feature 3088..3128 /label=BBa_B0012 terminator 3088..3128 /label=BBa_B0012 misc_feature 2212..3128 /label=BBa_J24704 misc_feature 1031..3128 /label=BBa_J24711 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaacctact agatggctga agcgcaaaat gatcccctgc tgccgggata 121 ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg gttatctcga 181 tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg 241 tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg atattttgct 301 gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca 361 ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat 421 atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct 481 gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat 541 aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc 601 accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag 661 caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca 721 tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgcatcag 781 ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt 841 tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc 901 gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcagc 961 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 1021 actactagag tcacacagga aacctactag atggtgaatg tgaaaccagt aacgttatac 1081 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc 1141 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac 1201 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc 1261 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc 1321 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt 1381 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg 1441 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt 1501 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg 1561 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc 1621 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc 1681 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa 1741 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat 1801 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat 1861 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc 1921 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc 1981 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa 2041 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 2101 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac 2161 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag 2221 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 2281 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 2341 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 2401 tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac 2461 cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 2521 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 2581 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 2641 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 2701 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 2761 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 2821 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 2881 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 2941 tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc 3001 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3061 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3121 cgtttata //