LOCUS BBa_J24724 3127 bp DNA linear 01-May-2024 DEFINITION 44. 16(BBa_J24694)==32(BBa_J24712) ACCESSION BBa_J24724 VERSION BBa_J24724.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 69..72 /label=conserved RBS 63..76 /label=BBa_B0031 CDS 83..958 /label=araC misc_feature 959..991 /label=LVA misc_feature 998..1022 /label=Help:Barcodes CDS 83..1022 /label=BBa_C0080 misc_feature 1..1022 /label=BBa_J24694 RBS 1031..1043 /label=RBS-3\Weak RBS 1037..1040 /label=RBS misc_feature 1031..1043 /label=BBa_B0032 RBS 1031..1043 /label=BBa_B0032 misc_feature 2139..2177 /label=LVA CDS 1050..2177 /label=lacI-LVA misc_feature 1050..2177 /label=BBa_C0012 misc_feature 2178..2202 /label=Help:Barcodes CDS 1050..2177 /label=BBa_C0012 misc_feature 1031..2202 /label=BBa_J24684 misc_feature 2215..2218 /label=conserved RBS 2211..2222 /label=BBa_B0034 CDS 2229..2990 /label=YFP (LVA) misc_feature 2985..2990 /label=2 misc_feature 2946..2984 /label=SsrA misc_feature 2470..2470 /label=A (Q->L) misc_feature 2229..2990 /label=BBa_E0032 misc_feature 2229..2990 /label=BBa_E0032 stem_loop 3010..3053 /label=stem_loop misc_feature 2999..3078 /label=BBa_B0010 terminator 2999..3078 /label=BBa_B0010 stem_loop 3094..3113 /label=T7 TE misc_feature 3120..3120 /label=stop polyA_site 3114..3127 /label=polya misc_feature 3087..3127 /label=BBa_B0012 terminator 3087..3127 /label=BBa_B0012 misc_feature 2211..3127 /label=BBa_J24704 misc_feature 1031..3127 /label=BBa_J24712 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaacctact agatggctga agcgcaaaat gatcccctgc tgccgggata 121 ctcgtttaac gcccatctgg tggcgggttt aacgccgatt gaggccaatg gttatctcga 181 tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg 241 tcagggggtg gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg atattttgct 301 gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca 361 ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat 421 atttgccaat acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct 481 gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat 541 aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc 601 accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag 661 caattttgat atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca 721 tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgcatcag 781 ccaggcgaag ctgcttttga gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt 841 tggttttgac gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc 901 gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcagc 961 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 1021 actactagag tcacacagga aagtactaga tggtgaatgt gaaaccagta acgttatacg 1081 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca 1141 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca 1201 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca 1261 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg 1321 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta 1381 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc 1441 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc 1501 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc 1561 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc 1621 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca 1681 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac 1741 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc 1801 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata 1861 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca 1921 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct 1981 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa 2041 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 2101 agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg 2161 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga 2221 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 2281 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 2341 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 2401 ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc 2461 acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 2521 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 2581 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 2641 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 2701 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 2761 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 2821 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 2881 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 2941 acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc 3001 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3061 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3121 gtttata //