LOCUS BBa_J24769 9938 bp DNA linear 14-May-2024 DEFINITION 89. 64(BBa_J24745) == 53(BBa_J24733) ACCESSION BBa_J24769 VERSION BBa_J24769.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 215..218 /label=conserved RBS 209..222 /label=BBa_B0031 CDS 229..1104 /label=araC misc_feature 1105..1137 /label=LVA misc_feature 1144..1168 /label=Help:Barcodes CDS 229..1168 /label=BBa_C0080 misc_feature 1..1168 /label=BBa_J24691 RBS 1177..1189 /label=RBS-3\Weak RBS 1183..1186 /label=RBS misc_feature 1177..1189 /label=BBa_B0032 RBS 1177..1189 /label=BBa_B0032 CDS 1199..1815 /label=tetR misc_feature 1816..1849 /label=SsrA misc_feature 1856..1880 /label=Help:Barcodes CDS 1196..1880 /label=BBa_C0040 misc_feature 1177..1880 /label=BBa_J24687 misc_feature 1893..1896 /label=conserved RBS 1889..1900 /label=BBa_B0034 CDS 1907..2614 /label=mCherry misc_feature 2258..2258 /label=C->T (removing PstI site) misc_feature 2615..2623 /label=LVA misc_feature 1907..2629 /label=BBa_J06505 stem_loop 2649..2692 /label=stem_loop misc_feature 2638..2717 /label=BBa_B0010 terminator 2638..2717 /label=BBa_B0010 stem_loop 2733..2752 /label=T7 TE misc_feature 2759..2759 /label=stop polyA_site 2753..2766 /label=polya misc_feature 2726..2766 /label=BBa_B0012 terminator 2726..2766 /label=BBa_B0012 misc_feature 1889..2766 /label=BBa_J24702 misc_feature 1177..2766 /label=BBa_J24706 misc_feature 1..2766 /label=BBa_J24715 misc_feature 2775..2793 /label=TetR 1 misc_feature 2775..2828 /label=BBa_R0040 promoter 2794..2799 /label=-35 misc_feature 2800..2818 /label=TetR 2 promoter 2817..2822 /label=-10 promoter 2775..2828 /label=BBa_R0040 misc_feature 2843..2846 /label=conserved RBS 2837..2850 /label=BBa_B0031 CDS 2857..3732 /label=araC misc_feature 3733..3765 /label=LVA misc_feature 3772..3796 /label=Help:Barcodes CDS 2857..3796 /label=BBa_C0080 misc_feature 2775..3796 /label=BBa_J24694 misc_feature 3811..3814 /label=conserved RBS 3805..3818 /label=BBa_B0031 misc_feature 4914..4952 /label=LVA CDS 3825..4952 /label=lacI-LVA misc_feature 3825..4952 /label=BBa_C0012 misc_feature 4953..4977 /label=Help:Barcodes CDS 3825..4952 /label=BBa_C0012 misc_feature 3805..4977 /label=BBa_J24683 misc_feature 4990..4993 /label=conserved RBS 4986..4997 /label=BBa_B0034 CDS 5004..5765 /label=YFP (LVA) misc_feature 5760..5765 /label=2 misc_feature 5721..5759 /label=SsrA misc_feature 5245..5245 /label=A (Q->L) misc_feature 5004..5765 /label=BBa_E0032 misc_feature 5004..5765 /label=BBa_E0032 stem_loop 5785..5828 /label=stem_loop misc_feature 5774..5853 /label=BBa_B0010 terminator 5774..5853 /label=BBa_B0010 stem_loop 5869..5888 /label=T7 TE misc_feature 5895..5895 /label=stop polyA_site 5889..5902 /label=polya misc_feature 5862..5902 /label=BBa_B0012 terminator 5862..5902 /label=BBa_B0012 misc_feature 4986..5902 /label=BBa_J24704 misc_feature 3805..5902 /label=BBa_J24711 misc_feature 2775..5902 /label=BBa_J24723 misc_feature 1..5902 /label=BBa_J24745 promoter 6990..7120 /label=Pbad promoter 6953..7024 /label=PC misc_feature 5911..6989 /label=AraC promoter 5911..7120 /label=BBa_I0500 misc_feature 7135..7138 /label=conserved RBS 7129..7142 /label=BBa_B0031 misc_feature 8238..8276 /label=LVA CDS 7149..8276 /label=lacI-LVA misc_feature 7149..8276 /label=BBa_C0012 misc_feature 8277..8301 /label=Help:Barcodes CDS 7149..8276 /label=BBa_C0012 misc_feature 7129..8301 /label=BBa_J24683 misc_feature 5911..8301 /label=BBa_J24697 RBS 8310..8322 /label=RBS-3\Weak RBS 8316..8319 /label=RBS misc_feature 8310..8322 /label=BBa_B0032 RBS 8310..8322 /label=BBa_B0032 CDS 8332..8948 /label=tetR misc_feature 8949..8982 /label=SsrA misc_feature 8989..9013 /label=Help:Barcodes CDS 8329..9013 /label=BBa_C0040 misc_feature 8310..9013 /label=BBa_J24687 misc_feature 9026..9029 /label=conserved RBS 9022..9033 /label=BBa_B0034 CDS 9040..9801 /label=CFP (LVA) misc_feature 9758..9795 /label=SsrA misc_feature 9796..9801 /label=2 misc_feature 9108..9108 /label=A misc_feature 9040..9801 /label=BBa_E0022 misc_feature 9040..9801 /label=BBa_E0022 stem_loop 9821..9864 /label=stem_loop misc_feature 9810..9889 /label=BBa_B0010 terminator 9810..9889 /label=BBa_B0010 stem_loop 9905..9924 /label=T7 TE misc_feature 9931..9931 /label=stop polyA_site 9925..9938 /label=polya misc_feature 9898..9938 /label=BBa_B0012 terminator 9898..9938 /label=BBa_B0012 misc_feature 9022..9938 /label=BBa_J24703 misc_feature 8310..9938 /label=BBa_J24709 misc_feature 5911..9938 /label=BBa_J24733 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggctgaagcg 241 caaaatgatc ccctgctgcc gggatactcg tttaacgccc atctggtggc gggtttaacg 301 ccgattgagg ccaatggtta tctcgatttt tttatcgacc gaccgctggg aatgaaaggt 361 tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg acgagaattt 421 gtctgccgac cgggtgatat tttgctgttc ccgccaggag agattcatca ctacggtcgt 481 catccggagg ctcgcgaatg gtatcaccag tgggtttact ttcgtccgcg cgcctactgg 541 catgaatggc ttaactggcc gtcaatattt gccaatacgg gtttctttcg cccggatgaa 601 gcgcaccagc cgcatttcag cgacctgttt gggcaaatca ttaacgccgg gcaaggggaa 661 gggcgctatt cggagctgct ggcgataaat ctgcttgagc aattgttact gcggcgcatg 721 gaagcgatta acgagtcgct ccatccaccg atggataatc gggtacgcga ggcttgtcag 781 tacatcagcg atcacctggc agacagcaat tttgatatcg ccagcgtcgc acagcatgtt 841 tgcctgtcgc cgtcgcgtct gtcacatctt ttccgccagc agttagggat tagcgtctta 901 agctggcgcg aggaccaacg catcagccag gcgaagctgc ttttgagcac tacccggatg 961 cctatcgcca ccgtcggtcg caatgttggt tttgacgatc aactctattt ctcgcgagta 1021 tttaaaaaat gcaccggggc cagcccgagc gagttccgtg ccggttgtga agaaaaagtg 1081 aatgatgtag ccgtcaagtt gtcagctgca aacgacgaaa actacgcttt agtagcttaa 1141 taacactgat agtgctagtg tagatcacta ctagagtcac acaggaaagt actagatgtc 1201 cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat 1261 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta 1321 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag 1381 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa 1441 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg 1501 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca 1561 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt 1621 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac 1681 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc 1741 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa 1801 atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg 1861 atagtgctag tgtagatcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1921 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1981 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 2041 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 2101 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 2161 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 2221 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 2281 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 2341 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2401 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2461 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2521 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2581 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagagcca 2641 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2701 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2761 tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata 2821 ctgagcacta ctagagtcac acaggaaacc tactagatgg ctgaagcgca aaatgatccc 2881 ctgctgccgg gatactcgtt taacgcccat ctggtggcgg gtttaacgcc gattgaggcc 2941 aatggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta tattctcaat 3001 ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt ctgccgaccg 3061 ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca tccggaggct 3121 cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca tgaatggctt 3181 aactggccgt caatatttgc caatacgggt ttctttcgcc cggatgaagc gcaccagccg 3241 catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg gcgctattcg 3301 gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga agcgattaac 3361 gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta catcagcgat 3421 cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg cctgtcgccg 3481 tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag ctggcgcgag 3541 gaccaacgca tcagccaggc gaagctgctt ttgagcacta cccggatgcc tatcgccacc 3601 gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgagtatt taaaaaatgc 3661 accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa tgatgtagcc 3721 gtcaagttgt cagctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag 3781 tgctagtgta gatcactact agagtcacac aggaaaccta ctagatggtg aatgtgaaac 3841 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 3901 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 3961 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 4021 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 4081 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 4141 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 4201 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 4261 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 4321 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 4381 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 4441 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 4501 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 4561 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 4621 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 4681 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 4741 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 4801 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 4861 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa 4921 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac 4981 tagagaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg 5041 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 5101 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 5161 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg 5221 cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct 5281 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 5341 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 5401 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 5461 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 5521 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 5581 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 5641 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 5701 gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt 5761 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 5821 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 5881 ggtgggcctt tctgcgttta tatactagag ttatgacaac ttgacggcta catcattcac 5941 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac 6001 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat 6061 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa 6121 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac 6181 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg 6241 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat 6301 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc 6361 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc 6421 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca 6481 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg 6541 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa 6601 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc 6661 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt 6721 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc 6781 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat 6841 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc 6901 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa 6961 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg 7021 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca 7081 actctctact gtttctccat acccgttttt ttgggctagc tactagagtc acacaggaaa 7141 cctactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 7201 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 7261 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 7321 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 7381 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 7441 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 7501 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 7561 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 7621 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 7681 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 7741 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 7801 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 7861 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 7921 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 7981 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 8041 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 8101 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 8161 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 8221 gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct 8281 gatagtgcta gtgtagatct ctactagagt cacacaggaa agtactagat gtccagatta 8341 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt 8401 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat 8461 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat 8521 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa 8581 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg 8641 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt 8701 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc 8761 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat 8821 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca 8881 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa 8941 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 9001 tagtgtagat cactactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag 9061 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag 9121 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 9181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg 9241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 9301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 9361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 9421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc 9481 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 9541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 9601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 9661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 9721 gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac 9781 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 9841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 9901 cactggctca ccttcgggtg ggcctttctg cgtttata //